Staar Stefanie, Richter Dagmar-Ulrike, Makovitzky Josef, Briese Volker, Bergemann Claudia
University of Rostock, Department of Obstetrics and Gynaecology, Faculty of Medicine, Doberaner Strasse 142, D-18055 Rostock, Germany.
Anticancer Res. 2005 May-Jun;25(3A):1713-8.
Phytoestrogens seem to have estrogen-like effects in the human body as their structure is very similar to those estrogens produced in human glands. The aim of the present study was to analyse the effects of genistein and daidzein on estrogen receptor (ER)alpha- and ERbeta-mRNA and protein expression in the endometrium of premenopausal women.
Glandular endometrial cells were isolated from endometrial biopsies obtained from regularly menstruating women undergoing gynaecological abrasio or hysterectomy. Cells were stimulated with single doses of genistein or daidzein. ERalpha- and ERbeta-protein expression were determined by immunocytochemical analysis. In addition ERalpha- and ERbeta-mRNA expression were determined by quantitative real-time RT-PCR. Quantification was carried out by the deltadelta C(T)-method using glyceraldehyde phosphate dehydrogenase (GAPDH) as housekeeping gene.
Endometrial glandular cells responded to stimulation with genistein and daidzein by alteration of both ERalpha- and ERbeta-mRNA expression. The effects were time- and dose-dependent. Detection of ERalpha- and ERbeta-protein expression by immunocytochemistry showed a dose-dependent regulation after stimulation of isolated endometrial cells with phytoestrogens.
According to our results, we suggest that ER expression in endometrial glandular cells is regulated by genistein and daidzein on the mRNA and protein levels. We could detect a decrease in ERalpha- and an increase in ERbeta-mRNA expression after stimulation with tested phytoestrogens. Our results are in line with findings that phytoestrogens act as anti-estrogens in organs expressing more ERalpha and as estrogens in ERbeta-presenting organs.
植物雌激素在人体中似乎具有类似雌激素的作用，因为它们的结构与人体腺体产生的雌激素非常相似。本研究的目的是分析染料木黄酮和大豆苷元对绝经前妇女子宫内膜中雌激素受体（ER）α和ERβ的mRNA及蛋白表达的影响。
从接受妇科刮宫术或子宫切除术的规律月经妇女的子宫内膜活检组织中分离出腺上皮细胞。用单剂量的染料木黄酮或大豆苷元刺激细胞。通过免疫细胞化学分析测定ERα和ERβ蛋白表达。此外，通过定量实时逆转录聚合酶链反应（RT-PCR）测定ERα和ERβ的mRNA表达。使用甘油醛-3-磷酸脱氢酶（GAPDH）作为管家基因，通过ΔΔC(T)法进行定量分析。
子宫内膜腺上皮细胞对染料木黄酮和大豆苷元的刺激有反应，ERα和ERβ的mRNA表达均发生改变。这些影响具有时间和剂量依赖性。用免疫细胞化学检测ERα和ERβ蛋白表达显示，在用植物雌激素刺激分离的子宫内膜细胞后呈现剂量依赖性调节。
根据我们的结果，我们认为染料木黄酮和大豆苷元在mRNA和蛋白水平上调节子宫内膜腺上皮细胞中的ER表达。在用受试植物雌激素刺激后，我们检测到ERα的mRNA表达减少，ERβ的mRNA表达增加。我们的结果与以下发现一致，即植物雌激素在表达更多ERα的器官中起抗雌激素作用，而在存在ERβ的器官中起雌激素作用。
