Kelly P A, Sharkey J, Ritchie I M
Department of Clinical Neurosciences, University of Edinburgh, Western General Hospital, Scotland, U.K.
Neuroscience. 1992;48(2):417-22. doi: 10.1016/0306-4522(92)90501-r.
The quasi-steady-state distribution volume of brain glucose was measured using 3-O-[14C]methylglucose quantitative autoradiography in a group of rats (n = 5) which 12-15 weeks previously had undergone unilateral ibotenic acid-induced lesion of the nucleus basalis magnocellularis, followed by implantation into ipsilateral neocortex of primordial basal forebrain cell suspensions. The effects of the lesion and the presence of transplanted tissue in neocortex were visualized by acetylcholinesterase histochemistry. The 3-O-[14C]methylglucose tracer was distributed homogeneously throughout the host brain areas analysed, with no side-to-side differences, despite a marked unilateral depletion of acetylcholinesterase activity ipsilateral to the lesion site. Whilst the transplants were indistinguishable from the homogeneity of surrounding host frontal cortex, there was a 70% increase in the apparent distribution volume of methylglucose localized around the ibotenate injection site and associated needle tract. Brain glucose content is an important experimental variable affecting the lumped constant of the 2-deoxyglucose technique. There was no evidence of any significant difference in the lumped constant between transplant and host tissue which might compromise the validity of the 2-deoxyglucose technique when used together with intracerebral implantation of fetal neuronal cell suspensions.
在一组大鼠（n = 5）中，使用3-O-[14C]甲基葡萄糖定量放射自显影法测量脑葡萄糖的准稳态分布容积。这些大鼠在12 - 15周前接受了单侧鹅膏蕈氨酸诱导的大细胞基底核损伤，随后将原始基底前脑细胞悬液植入同侧新皮质。通过乙酰胆碱酯酶组织化学观察损伤和新皮质中移植组织的存在情况。尽管损伤部位同侧的乙酰胆碱酯酶活性明显单侧耗竭，但3-O-[14C]甲基葡萄糖示踪剂在整个分析的宿主脑区中分布均匀，没有左右差异。虽然移植组织与周围宿主额叶皮质的均匀性无法区分，但在鹅膏蕈氨酸注射部位及相关针道周围，甲基葡萄糖的表观分布容积增加了70%。脑葡萄糖含量是影响2-脱氧葡萄糖技术集总常数的一个重要实验变量。在移植组织和宿主组织之间，没有证据表明集总常数存在任何显著差异，这可能会损害2-脱氧葡萄糖技术与胎儿神经元细胞悬液脑内植入联合使用时的有效性。
