Molero Laura, García-Méndez Antonio, Alonso-Orgaz Sergio, Carrasco Carolina, Macaya Carlos, López Farré Antonio J
Cardiovascular Research Unit, Cardiovascular Institute, Hospital Clínico San Carlos, C/Profesor Martín Lagos s/n, Madrid 28040, Spain.
Clin Sci (Lond). 2005 Nov;109(5):457-63. doi: 10.1042/CS20050082.
The aim of the present study was to use proteomics to analyse modifications in the level of expression of different proteins in BVSMCs (bovine vascular smooth muscle cells) incubated in the absence and presence of 17beta-oestradiol. By using two-dimensional electrophoresis with a pH range of 4-7, we identified several areas on the gels in which the level of expression of proteins were different between control BVSMCs and cells incubated for 24 h with 17beta-oestradiol. Changes in several isoforms of alpha-enolase, HSP60 (heat-shock protein 60), vimentin and PDI (protein disulphide-isomerase) were observed in BVSMCs. The expression of alpha-enolase isoform 1 was enhanced after 17beta-oestradiol treatment. The expression of HSP60 isoform 3, vimentin isoforms 2 and 3 and caldesmon was reduced by 17beta-oestradiol. Finally, the expression of PDI isoforms was reduced by 17beta-oestradiol. In summary, 17beta-oestradiol modified the expression of isoforms of proteins associated with smooth muscle cell proliferation (alpha-enolase, vimentin and HSP-60), cell contraction (vimentin and caldesmon) and cell redox modulation (PDI). These findings confirm that 17beta-oestradiol may modulate a wide range of signalling pathways in vascular smooth muscle cells.
本研究的目的是利用蛋白质组学分析在不存在和存在17β-雌二醇的情况下培养的牛血管平滑肌细胞（BVSMCs）中不同蛋白质表达水平的变化。通过使用pH范围为4 - 7的二维电泳，我们在凝胶上鉴定出几个区域，其中对照BVSMCs与用17β-雌二醇培养24小时的细胞之间蛋白质的表达水平不同。在BVSMCs中观察到α-烯醇化酶、热休克蛋白60（HSP60）、波形蛋白和蛋白二硫键异构酶（PDI）的几种同工型发生了变化。17β-雌二醇处理后，α-烯醇化酶同工型1的表达增强。17β-雌二醇使HSP60同工型3、波形蛋白同工型2和3以及钙调蛋白的表达降低。最后，17β-雌二醇使PDI同工型的表达降低。总之，17β-雌二醇改变了与平滑肌细胞增殖（α-烯醇化酶、波形蛋白和HSP - 60）、细胞收缩（波形蛋白和钙调蛋白）以及细胞氧化还原调节（PDI）相关的蛋白质同工型的表达。这些发现证实17β-雌二醇可能调节血管平滑肌细胞中的多种信号通路。
