MacDonald Kelli P A, Rowe Vanessa, Bofinger Helen M, Thomas Ranjeny, Sasmono Tedjo, Hume David A, Hill Geoffrey R
Bone Marrow Transplantation Laboratory, Queensland Institute of Medical Research, Queensland, Australia.
J Immunol. 2005 Aug 1;175(3):1399-405. doi: 10.4049/jimmunol.175.3.1399.
The lineage of dendritic cells (DC), and in particular their relationship to monocytes and macrophages, remains obscure. Furthermore, the requirement for the macrophage growth factor CSF-1 during DC homeostasis is unclear. Using a transgenic mouse in which the promoter for the CSF-1R (c-fms) directs the expression of enhanced GFP in cells of the myeloid lineage, we determined that although the c-fms promoter is inactive in DC precursors, it is up-regulated in all DC subsets during differentiation. Furthermore, plasmacytoid DC and all CD11c(high) DC subsets are reduced by 50-70% in CSF-1-deficient osteopetrotic mice, confirming that CSF-1 signaling is required for the optimal differentiation of DC in vivo. These data provide additional evidence that the majority of tissue DC is of myeloid origin during steady state and supports a close relationship between DC and macrophage biology in vivo.
树突状细胞（DC）的谱系，尤其是它们与单核细胞和巨噬细胞的关系，仍然不清楚。此外，在DC稳态过程中对巨噬细胞生长因子CSF-1的需求也不明确。利用一种转基因小鼠，其中CSF-1R（c-fms）的启动子指导髓系谱系细胞中增强型GFP的表达，我们确定虽然c-fms启动子在DC前体细胞中无活性，但在分化过程中所有DC亚群中它都会上调。此外，在CSF-1缺陷的骨硬化小鼠中，浆细胞样DC和所有CD11c（高）DC亚群减少了50-70%，证实CSF-1信号对于体内DC的最佳分化是必需的。这些数据提供了额外的证据，表明在稳态期间大多数组织DC起源于髓系，并支持体内DC与巨噬细胞生物学之间的密切关系。
