Nagaoka S, Satoh T, Sakamoto K, Ihara H
Kumamoto Industrial Research Institute, Higashi-machi, Kumamoto 862-0901, Japan.
J Chromatogr A. 2005 Aug 5;1082(2):185-92. doi: 10.1016/j.chroma.2005.05.063.
Porous polymer spherical particles for column packings in nonaqueous size-exclusion chromatography (SEC) were prepared from 1,2-syndiotactic polybutadiene by suspension and evaporation method. The polymer microbeads obtained were crosslinked by radical reaction between 2-vinyl groups in polybutadiene with ultraviolet radiation, to render them insoluble. These microbeads have wider chromatographic separation width than polystyrene column packings. In addition, the polybutadiene microbeads did not show the excessive retention observed with commercial polystyrene columns for polycyclic aromatic compounds. Therefore, a close correlation between the elution volume and M, for polycyclic aromatic compounds was observed with polybutadiene microbeads columns.
采用悬浮蒸发法，以1,2-间同立构聚丁二烯为原料制备了用于非水尺寸排阻色谱（SEC）柱填料的多孔聚合物球形颗粒。通过聚丁二烯中2-乙烯基之间的自由基反应，利用紫外线辐射对得到的聚合物微珠进行交联，使其不溶。这些微珠比聚苯乙烯柱填料具有更宽的色谱分离宽度。此外，聚丁二烯微珠对于多环芳烃并未表现出商业聚苯乙烯柱所观察到的过度保留现象。因此，在聚丁二烯微珠柱上观察到多环芳烃的洗脱体积与M之间存在密切相关性。
