Reverberi Massimo, Fanelli Corrado, Zjalic Slaven, Briganti Stefania, Picardo Mauro, Ricelli Alessandra, Fabbri Anna Adele
Dipartimento Biologia Vegetale, Università La Sapienza, Largo Cristina di Svezia, 24, Rome, 00165, Italy.
Free Radic Res. 2005 Jun;39(6):637-47. doi: 10.1080/10715760500072115.
Plant responses to biotic and abiotic stress can be mediated by oxidised products and in this study we analysed the relation among some of them and the growth factor indole-3-acetic acid (IAA). The plant material used was potato tuber sliced below bud and incubated for different lengths of time before analysis. Wounding in potato tuber leads, in a very short time (0-30?min), to the generation of lipid hydroperoxides (LOOH) from polyunsaturated fatty acids (PUFA). These reactive species could cause a subsequent increase of 9 and 13-lipoxygenase (LOX, E.C.1.13.12.12.), analysed by RT-PCR and spectrophotometric assay, LOOH, Jasmonates and IAA all quantified by GC-MS analysis. The activation of 9 and 13-LOX, using different timing, leads to the formation of LOOH with a subsequent generation of jasmonates and IAA as highlighted by the addition on the potato tuber slices of salicylhydroxamic acid (SHAM), an inhibitor of LOX activity. A correlation between jasmonates and IAA resulted by testing their reciprocal influence during wounding in potato tuber. The relationship occurring among each hormone analysed during wounding underlines the fact that the jasmonates level can be regulated in situ and this can suggest a role for these compounds in potato tuber which has been underestimated up to now.
植物对生物和非生物胁迫的响应可由氧化产物介导，在本研究中，我们分析了其中一些氧化产物与生长因子吲哚 - 3 - 乙酸（IAA）之间的关系。所用的植物材料是在芽下切片的马铃薯块茎，在分析前孵育不同的时间长度。马铃薯块茎受伤后，在很短的时间内（0 - 30分钟），多不饱和脂肪酸（PUFA）会生成脂质氢过氧化物（LOOH）。这些活性物质可能会导致9和13 - 脂氧合酶（LOX，E.C.1.13.12.12.）随后增加，通过RT - PCR和分光光度法测定，LOOH、茉莉酸酯和IAA均通过GC - MS分析进行定量。使用不同的时间点激活9和13 - LOX，会导致LOOH的形成，随后生成茉莉酸酯和IAA，水杨酸肟酸（SHAM）是一种LOX活性抑制剂，添加到马铃薯块茎切片上就突出了这一点。通过测试它们在马铃薯块茎受伤过程中的相互影响，得出了茉莉酸酯与IAA之间的相关性。受伤过程中分析的每种激素之间的关系强调了这样一个事实，即茉莉酸酯水平可以原位调节，这可能表明这些化合物在马铃薯块茎中的作用迄今为止一直被低估。
