抗人白细胞5-脂氧合酶单克隆抗体的特性鉴定

Characterization of monoclonal antibodies against human leukocyte 5-lipoxygenase.

作者信息

Ethier D, Evans J F

机构信息

Department of Pharmacology, Merck Frosst Centre for Therapeutic Research, Pointe Claire-Dorval, Quebec, Canada.

出版信息

Prostaglandins Leukot Essent Fatty Acids. 1992 Apr;45(4):259-65. doi: 10.1016/0952-3278(92)90081-s.

DOI:10.1016/0952-3278(92)90081-s

PMID:1603817

Abstract

Four mouse monoclonal IgG1 antibody-producing cell lines (5LO-1, 5LO-2, 5LO-3, 5LO-4), produced against highly purified human leukocyte 5-lipoxygenase have been characterized. The monoclonal antibodies produced by these cell lines exhibited differential reactivity against 5-lipoxygenase as determined by ELISA and immunoprecipitation analyses. Monoclonal antibodies 5LO-2 and 5LO-3 inhibited the activity of recombinant human leukocyte 5-lipoxygenase in a dose-dependent manner. This inhibition was selective for 5-lipoxygenase activity since these monoclonal antibodies did not inhibit human leukocyte 15-lipoxygenase or porcine leukocyte 12-lipoxygenase.

摘要

已经鉴定了四种针对高度纯化的人白细胞5-脂氧合酶产生的小鼠单克隆IgG1抗体产生细胞系（5LO-1、5LO-2、5LO-3、5LO-4）。通过ELISA和免疫沉淀分析确定，这些细胞系产生的单克隆抗体对5-脂氧合酶表现出不同的反应性。单克隆抗体5LO-2和5LO-3以剂量依赖性方式抑制重组人白细胞5-脂氧合酶的活性。由于这些单克隆抗体不抑制人白细胞15-脂氧合酶或猪白细胞12-脂氧合酶，因此这种抑制对5-脂氧合酶活性具有选择性。