Veledo Maria Teresa, de Frutos Mercedes, Diez-Masa Jose Carlos
Institute of Organic Chemistry (C.S.I.C.), Juan de la Cierva 3, 28006 Madrid, Spain.
J Chromatogr A. 2005 Jun 24;1079(1-2):335-43. doi: 10.1016/j.chroma.2005.03.111.
Free amino acids have been derivatized on-capillary with 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ) and analyzed using a laboratory-made capillary electrophoresis apparatus with laser-induced fluorescence detection. Several parameters that control on-capillary derivatization of amino acids, including pH, mixing time, reaction time, concentration of the derivatization reagents (potassium cyanide and FQ) and solvent of FQ, as well as the temperature of mixing and reaction were optimized. Repeatabilities better than 1.8% for migration time and 7.8% for peak height were obtained. Assay detection limits for the different amino acids ranged from 23 nM for glycine to 50 nM for lysine and glutamic acid. The methods developed were applied to the analysis of several amino acids in pharmaceutical preparations and plasma samples. Results showed a good agreement with those obtained using an amino acid autoanalyzer for the same samples.
游离氨基酸已在毛细管上用3-(2-呋喃甲酰基)喹啉-2-甲醛(FQ)进行衍生化，并使用自制的带有激光诱导荧光检测的毛细管电泳仪进行分析。对控制氨基酸在毛细管上衍生化的几个参数进行了优化，包括pH值、混合时间、反应时间、衍生化试剂(氰化钾和FQ)的浓度、FQ的溶剂以及混合和反应温度。迁移时间的重复性优于1.8%，峰高的重复性优于7.8%。不同氨基酸的测定检测限范围从甘氨酸的23 nM到赖氨酸和谷氨酸的50 nM。所开发的方法应用于药物制剂和血浆样品中几种氨基酸的分析。结果表明，与使用氨基酸自动分析仪对相同样品获得的结果具有良好的一致性。
