[神经干细胞移植对大鼠脊髓损伤后胶质细胞源性神经营养因子及生长相关蛋白43的影响]

[Effects of neural stem cells transplantation on glial cell line-derived neurotrophic factor and growth associated protein 43 after spinal cord injury in rats].

作者信息

Wang Yanfeng, Lu Gang, Li Lei, Han Zhuang, Yang Maowei, Huang Tao

机构信息

Department of Orthopaedics, the First Affiliated Hospital, China Medical University, Shenyang Liaoning, 110001, P. R. China.

出版信息

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 Jun;19(6):416-9.

PMID:16038451

Abstract

OBJECTIVE

To observe the effects of neural stem cells(NSCs) transplantation on the glial cell line-derived neurotrophic factor (GDNF) and growth associated protein 43(GAP-43) after the spinal cord injury (SCI), and to investigate the mechanism of repairing the SCI by NSCs transplantation.

METHODS

The neural stem cells from the hippocampus of rats' embryo were cultured and identified by immunocytochemistry. The SCI model was made by the modified Allen device. Sixty adult Wistar rats were randomly divided into three groups: spinal cord injury was treated with transplantation of NSCs (group A, n = 24), with DMEM solution (group B, n = 24) and normal control group without being injured(group C, n = 12). Seven days after the operation of SCI, the NSCs were transplanted into the injured site. Then GAP-43 and GDNF expressions were tested by RT-PCR and immunohistochemistry.

RESULTS

Compared with group B, the GDNF mRNA expression of group A increased by 23.3% on the 1st day, by 26.8% on the 3rd day and by 32.7% on the 7th day; the GAP-43 mRNA expression increased by 19.5% on the 1st day, 21.6% on the 3rd day and 23.1% on the 7th day. There were statistically significant differences (P<0.05).

CONCLUSION

The transplantation of NSCs can change the micro-environment injured site and promote the regeneration of axon by enhancing the expressions of GDNF mRNA and GAP-43 mRNA. It is one of the mechanisms of repairing the SCI by NSCs transplantation.

摘要

目的

观察神经干细胞（NSCs）移植对脊髓损伤（SCI）后胶质细胞源性神经营养因子（GDNF）和生长相关蛋白43（GAP - 43）的影响，探讨NSCs移植修复SCI的机制。

方法

培养大鼠胚胎海马神经干细胞并进行免疫细胞化学鉴定。采用改良Allen装置制备SCI模型。60只成年Wistar大鼠随机分为3组：NSCs移植治疗脊髓损伤组（A组，n = 24）、DMEM溶液治疗组（B组，n = 24）和未损伤正常对照组（C组，n = 12）。SCI手术后7天，将NSCs移植到损伤部位。然后通过RT - PCR和免疫组织化学检测GAP - 43和GDNF表达。