Traversa Donato, Otranto Domenico, Iorio Raffaella, Giangaspero Annunziata
Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro 45, 64100 Teramo, Italy.
Mol Cell Probes. 2005 Aug;19(4):245-9. doi: 10.1016/j.mcp.2005.01.005. Epub 2005 Apr 18.
Equine thelaziosis caused by the eyeworm Thelazia lacrymalis is a parasitic disease transmitted by muscid flies. Although equine thelaziosis is known to have worldwide distribution, information on the epidemiology and presence of the intermediate hosts of T. lacrymalis is lacking. In the present work, a PCR-RFLP based assay on the first and/or second internal transcribed spacer (ITS1 and ITS2) of ribosomal DNA was developed for the detection of T. lacrymalis DNA in its putative vector(s). The sensitivity of the technique was also assessed. The restriction patterns obtained readily differentiated T. lacrymalis from four species of Musca (Diptera, Muscidae) (i.e. Musca autumnalis, Musca domestica, Musca larvipara and Musca osiris), which are potential vectors of equine eyeworms. The molecular assay presented herein is a useful tool to identify the intermediate host(s) of T. lacrymalis in natural conditions and to study its/their ecology and epidemiology.
由泪管吸吮线虫（Thelazia lacrymalis）引起的马吸吮线虫病是一种由蝇科苍蝇传播的寄生虫病。尽管已知马吸吮线虫病在全球范围内均有分布，但关于泪管吸吮线虫中间宿主的流行病学及存在情况的信息却十分匮乏。在本研究中，我们开发了一种基于核糖体DNA第一和/或第二内部转录间隔区（ITS1和ITS2）的PCR-RFLP分析方法，用于检测泪管吸吮线虫在其假定传播媒介中的DNA。同时还评估了该技术的灵敏度。所获得的限制性酶切图谱能够轻松地将泪管吸吮线虫与四种家蝇属（双翅目，蝇科）（即秋家蝇、家蝇、幼虫家蝇和埃及家蝇）区分开来，这些家蝇均为马眼虫的潜在传播媒介。本文介绍的分子检测方法是一种有用的工具，可用于在自然条件下鉴定泪管吸吮线虫的中间宿主，并研究其生态和流行病学。
