Ohguchi Atsuko, Nakayama Yumi, Yasoshima Akira, Doi Chiyo, Mikami Takashi, Uetsuka Koji, Nakayama Hiroyuki, Doi Kunio
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Exp Mol Pathol. 2006 Apr;80(2):201-7. doi: 10.1016/j.yexmp.2005.06.003. Epub 2005 Jul 20.
Development of acinar cell apoptosis and ultrastructural changes in the exorbital lacrimal and parotid glands was examined in DBA/2 mice infected with 10(2) PFU/mouse of EMC-D virus. Pyknotic acinar cells, most of which were positive for TUNEL and cleaved caspase-3 and had ultrastructural characteristics of apoptotic cells, developed earlier and were more frequently observed in the parotid gland than in the exorbital lacrimal gland, while the total damage of acinar cells and interstitial infiltration of macrophages were more prominent in the latter than in the former. These findings indicate that EMC-D virus induces acinar cell apoptosis in these glands. In addition, corresponding to the results of the detection of viral RNA signals by in situ hybridization, small aggregates of virus-like particles having typical size and structure of EMC virus were frequently observed in both the cytoplasm and the nucleus of acinar cells in the exorbital lacrimal gland, while they were found only in the cytoplasm of a few acinar cells in the parotid gland. In conclusion, between the exorbital lacrimal and parotid glands, there was a reverse relationship observed between the development of acinar cell apoptosis and that of total damage of acinar cells.
在感染了10(2) PFU/小鼠的EMC-D病毒的DBA/2小鼠中，研究了眶外泪腺和腮腺中腺泡细胞凋亡的发展以及超微结构变化。固缩的腺泡细胞，其中大多数TUNEL和裂解的caspase-3呈阳性，并具有凋亡细胞的超微结构特征，在腮腺中比在眶外泪腺中出现得更早且更频繁地被观察到，而腺泡细胞的总体损伤和巨噬细胞的间质浸润在后者比前者更明显。这些发现表明EMC-D病毒在这些腺体中诱导腺泡细胞凋亡。此外，对应于原位杂交检测病毒RNA信号的结果，在眶外泪腺的腺泡细胞的细胞质和细胞核中经常观察到具有EMC病毒典型大小和结构的病毒样颗粒小聚集体，而在腮腺中仅在少数腺泡细胞的细胞质中发现它们。总之，在眶外泪腺和腮腺之间，腺泡细胞凋亡的发展与腺泡细胞总体损伤的发展之间存在相反的关系。
