Skupień Katarzyna, Oszmiański Jan, Kostrzewa-Nowak Dorota, Tarasiuk Jolanta
Department of Processing and Storage of Plant Raw Materials, Agricultural Academy of Szczecin, 17 Słowackiego st, 71-434 Szczecin, Poland.
Cancer Lett. 2006 May 18;236(2):282-91. doi: 10.1016/j.canlet.2005.05.018. Epub 2005 Jul 20.
The aim of the present study was to determine in vitro antileukaemic activity of extracts obtained from selected berry plant leaves (Fragaria x ananassa Duch. cv Elsanta, raspberry Rubus ideus L. cv Polana and blueberry Vaccinium corymbosum L. cv Bluecrop) against promyelocytic HL60 cell line and its multidrug resistant sublines exhibiting two different MDR phenotypes: HL60/VINC (overexpressing P-glycoprotein) and HL60/DOX (overexpressing MRP1 protein). It was found that the blueberry extract was the most efficient against sensitive HL60 cell line (about 2-fold more active than strawberry and raspberry extracts) but presented much lower activity towards resistant cells. In contrast, strawberry and raspberry extracts exhibited the high cytotoxic activity against sensitive leukaemia HL60 cell line as well as its MDR sublines. The values of resistance factor (RF) found for these extracts were very low lying in the range 0.32/2.0.
本研究的目的是测定从选定浆果植物叶片（草莓品种Elsanta、树莓品种Polana和蓝莓品种Bluecrop）中提取的提取物对早幼粒细胞HL60细胞系及其表现出两种不同多药耐药表型的多药耐药亚系的体外抗白血病活性：HL60/VINC（过表达P-糖蛋白）和HL60/DOX（过表达MRP1蛋白）。结果发现，蓝莓提取物对敏感的HL60细胞系最有效（活性比草莓和树莓提取物高约2倍），但对耐药细胞的活性要低得多。相比之下，草莓和树莓提取物对敏感白血病HL60细胞系及其多药耐药亚系均表现出高细胞毒性活性。这些提取物的耐药因子（RF）值非常低，在0.32/2.0范围内。
