Ciafrè S A, Galardi S, Mangiola A, Ferracin M, Liu C-G, Sabatino G, Negrini M, Maira G, Croce C M, Farace M G
Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Roma, Italy.
Biochem Biophys Res Commun. 2005 Sep 9;334(4):1351-8. doi: 10.1016/j.bbrc.2005.07.030.
MicroRNAs (miRNAs) are short non-coding RNA molecules playing regulatory roles in animals and plants by repressing translation or cleaving RNA transcripts. The specific modulation of several microRNAs has been recently associated to some forms of human cancer, suggesting that these short molecules may represent a new class of genes involved in oncogenesis. In our study, we examined by microarray the global expression levels of 245 microRNAs in glioblastoma multiforme, the most frequent and malignant of primary brain tumors. The analysis of both glioblastoma tissues and glioblastoma cell lines allowed us to identify a group of microRNAs whose expression is significantly altered in this tumor. The most interesting results came from miR-221, strongly up-regulated in glioblastoma and from a set of brain-enriched miRNAs, miR-128, miR-181a, miR-181b, and miR-181c, which are down-regulated in glioblastoma.
微小RNA（miRNA）是短的非编码RNA分子，通过抑制翻译或切割RNA转录本在动植物中发挥调节作用。最近，几种微小RNA的特定调节作用已与某些形式的人类癌症相关联，这表明这些短分子可能代表了一类参与肿瘤发生的新基因。在我们的研究中，我们通过微阵列检测了多形性胶质母细胞瘤（原发性脑肿瘤中最常见且恶性程度最高的肿瘤）中245种微小RNA的整体表达水平。对胶质母细胞瘤组织和胶质母细胞瘤细胞系的分析使我们能够鉴定出一组在该肿瘤中表达显著改变的微小RNA。最有趣的结果来自在胶质母细胞瘤中强烈上调的miR-221，以及一组在胶质母细胞瘤中下调的脑富集miRNA，即miR-128、miR-181a、miR-181b和miR-181c。
