银狐（赤狐）和蓝狐（北极狐）精子膜脂肪酸组成的比较研究及其与细胞冷冻保存的关系。

Comparative aspects of sperm membrane fatty acid composition in silver (Vulpes vulpes) and blue (Alopex lagopus) foxes, and their relationship to cell cryopreservation.

作者信息

Miller R R, Cornett C L, Waterhouse K E, Farstad W

机构信息

Hillsdale College, Biology Department, 33 E. College, Hillsdale, MI 49242, USA.

出版信息

Cryobiology. 2005 Aug;51(1):66-75. doi: 10.1016/j.cryobiol.2005.04.009.

DOI:10.1016/j.cryobiol.2005.04.009

PMID:16040024

Abstract

Cryogenic protocols have been developed for the storage of farmed silver fox (Vulpes vulpes) spermatozoa. However, these same protocols and modifications of these protocols have failed to satisfactorily preserve spermatozoa collected from farmed blue foxes (Alopex lagopus). Because cryogenic success has been linked to membrane composition, the plasma membrane lipid composition of farmed blue fox and silver fox spermatozoa was studied. Silver fox spermatozoal membranes have significantly higher levels of docosapentaenoic acid (DPA; 22:5, n-6) compared to blue fox spermatozoa, and blue fox spermatozoal membranes have significantly higher levels of stearic acid (18:0). Silver fox spermatozoal membranes not only have a higher ratio of unsaturated/saturated membrane fatty acids, but also higher levels of membrane desmosterol and cholesterol.

摘要

已经开发出用于养殖银狐（赤狐）精子储存的低温保存方案。然而，这些相同的方案以及对这些方案的修改都未能令人满意地保存从养殖蓝狐（北极狐）采集的精子。由于低温保存的成功与膜组成有关，因此对养殖蓝狐和银狐精子的质膜脂质组成进行了研究。与蓝狐精子相比，银狐精子膜中的二十二碳五烯酸（DPA；22:5，n-6）水平显著更高，而蓝狐精子膜中的硬脂酸（18:0）水平显著更高。银狐精子膜不仅具有更高的不饱和/饱和膜脂肪酸比例，而且具有更高水平的膜脱胆固醇和胆固醇。