全反式维甲酸使DNA疫苗诱导的免疫反应向Th2方向偏移。

All-trans retinoic acid biases immune response induced by DNA vaccine in a Th2 direction.

作者信息

Yu Sanhong, Xia Mingcan, Xu Wei, Chu Yiwei, Wang Ying, Xiong Sidong

机构信息

Department of Immunology, Shanghai Medical College, Fudan University, Shanghai, 200032, P.R. China.

出版信息

Vaccine. 2005 Oct 25;23(44):5160-7. doi: 10.1016/j.vaccine.2005.06.019.

DOI:10.1016/j.vaccine.2005.06.019

PMID:16040168

Abstract

Vitamin A deficiency diminishes Th2-mediated Ab responses. Providing Vitamin A or its active metabolites reverses this defect. All-trans retinoic acid (ATRA), an acid derivation of Vitamin A, regulates the balance of immune response induced by TR421-hCGbeta DNA vaccine. Compared to DNA vaccine alone or treatment with vehicle, significantly higher level of antibody against the protein encoded by DNA vaccine was observed in mice 6 weeks after the first immunization. The IgG2a/IgG1 ratio was lower in mice treated with ATRA. We also found that treatment with ATRA also diminishes specific cellular immune response induced by gene immunization by measuring the marker of cellular immune response. We conclude that ATRA biases the immune response to Th2 direction induced by DNA vaccine and acts as a candidate adjuvant and immunomodulatory molecule.

摘要

维生素A缺乏会削弱Th2介导的抗体反应。补充维生素A或其活性代谢产物可逆转这一缺陷。全反式维甲酸（ATRA）是维生素A的一种酸性衍生物，可调节TR421-hCGβ DNA疫苗诱导的免疫反应平衡。与单独使用DNA疫苗或使用赋形剂处理相比，在首次免疫6周后的小鼠中，观察到针对DNA疫苗编码蛋白的抗体水平显著更高。用ATRA处理的小鼠中IgG2a/IgG1比值较低。我们还发现，通过测量细胞免疫反应标志物，用ATRA处理也会削弱基因免疫诱导的特异性细胞免疫反应。我们得出结论，ATRA使DNA疫苗诱导的免疫反应偏向Th2方向，可作为候选佐剂和免疫调节分子。