溶血曼氏杆菌白细胞毒素在牛CD18内结合位点的定位
Mapping of the binding site for Mannheimia haemolytica leukotoxin within bovine CD18.
作者信息
Dileepan T, Kannan M S, Walcheck B, Thumbikat P, Maheswaran S K
机构信息
Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA.
出版信息
Infect Immun. 2005 Aug;73(8):5233-7. doi: 10.1128/IAI.73.8.5233-5237.2005.
Abstract
To map the site involved in Mannheimia haemolytica leukotoxin (LktA) binding and biological activity within bovine CD18, bovine x human CD18 chimeric constructs were generated and coexpressed with bovine CD11a in K562 cells. Studies with the chimeric leukocyte function-associated antigen 1 transductants demonstrate that the site required for LktA binding and biological effects resides within amino acid residues 500 and 600 of the extracellular region of bovine CD18.
摘要
为了定位溶血曼氏杆菌白细胞毒素（LktA）与牛CD18结合及生物活性相关的位点，构建了牛与人CD18的嵌合构建体，并在K562细胞中与牛CD11a共表达。对嵌合白细胞功能相关抗原1转导子的研究表明，LktA结合及生物效应所需的位点位于牛CD18胞外区氨基酸残基500至600之间。
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本文引用的文献
1
Characterization of the caprine (Capra hircus) beta-2 integrin CD18-encoding cDNA and identification of mutations potentially responsible for the ruminant-specific virulence of Mannheimia haemolytica.
Mol Membr Biol. 2004 Sep-Oct;21(5):289-95. doi: 10.1080/09687680412331282785.
2
Creating random mutagenesis libraries by megaprimer PCR of whole plasmid (MEGAWHOP).
Methods Mol Biol. 2003;231:23-8. doi: 10.1385/1-59259-395-X:23.
3
Role of Mannheimia haemolytica leukotoxin in the pathogenesis of bovine pneumonic pasteurellosis.
Anim Health Res Rev. 2002 Dec;3(2):69-82. doi: 10.1079/ahrr200242.
4
Intracellular calcium signaling through the cADPR pathway is agonist specific in porcine airway smooth muscle.
FASEB J. 2003 Mar;17(3):482-4. doi: 10.1096/fj.02-0622fje. Epub 2003 Jan 22.
5
Creating random mutagenesis libraries using megaprimer PCR of whole plasmid.
Biotechniques. 2002 Nov;33(5):1033-4, 1036-8. doi: 10.2144/02335st03.
6
Bovine CD18 is necessary and sufficient to mediate Mannheimia (Pasteurella) haemolytica leukotoxin-induced cytolysis.
Infect Immun. 2002 Sep;70(9):5058-64. doi: 10.1128/IAI.70.9.5058-5068.2002.
7
Response of the ruminant respiratory tract to Mannheimia (Pasteurella) haemolytica.
Microbes Infect. 2000 Jul;2(9):1079-88. doi: 10.1016/s1286-4579(00)01262-4.
8
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9
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