从生物转化溶液中回收丙酮酸。
Recovery of pyruvic acid from biotransformation solutions.
作者信息
Ma C Q, Li J C, Qiu J H, Wang M, Xu P
机构信息
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
出版信息
Appl Microbiol Biotechnol. 2006 Apr;70(3):308-14. doi: 10.1007/s00253-005-0072-0. Epub 2005 Jul 23.
The aim of this investigation was to separate pyruvic acid of biotransformation solutions from lactic acid through complex extraction. For this purpose, complex extraction was investigated from model solutions. Tri-n-octanylamine (TOA) was used as the extractant. The effects of various diluents, the stoichiometry of pyruvic acid to TOA, and the initial pH of the aqueous phase on the extraction process were investigated in this study. The effects of sodium hydroxide (NaOH) and trimethylamine (TMA) on the back extraction process were also studied, respectively. The optimal conditions attained from the model solutions proved efficient on the biotransformation solutions of different concentrations. A total recovery of 71-82% of pyruvic acid was obtained, whereas 89-92% of lactic acid was removed. The purity of pyruvic acid reached 97% after the removal of TMA by a simple distillation.
本研究的目的是通过络合萃取从生物转化溶液中分离丙酮酸和乳酸。为此，对模型溶液进行了络合萃取研究。使用三正辛胺（TOA）作为萃取剂。本研究考察了各种稀释剂、丙酮酸与TOA的化学计量比以及水相初始pH对萃取过程的影响。还分别研究了氢氧化钠（NaOH）和三甲胺（TMA）对反萃取过程的影响。从模型溶液中获得的最佳条件在不同浓度的生物转化溶液中证明是有效的。丙酮酸的总回收率达到71-82%，而乳酸的去除率为89-92%。通过简单蒸馏除去TMA后，丙酮酸的纯度达到97%。
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