Hahn Mariah S, Kobler James B, Zeitels Steven M, Langer Robert
Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Ann Otol Rhinol Laryngol. 2005 Jun;114(6):451-62. doi: 10.1177/000348940511400607.
We examined the proteoglycan (PG) and associated sulfated glycosaminoglycan (GAG) content of the midmembranous vocal fold lamina propria (LP) of humans, dogs, pigs, and ferrets.
The LP PG levels were assessed indirectly by quantifying the associated sulfated GAGs, and immunohistochemical analyses of specific PGs and/or GAGs (PGs/GAGs) were conducted.
Sulfated GAGs constituted approximately (average +/- SEM) 14.7 +/- 2.1 microg per milligram of tissue total protein in the human LP--similar to levels in canine, porcine, and ferret LPs (p > .05). Immunohistochemical analysis identified versican, chondroitin 4- and 6-sulfate, and heparan sulfate in the LP extracellular matrix--PGs/GAGs previously believed to be localized only intracellularly and in the basement membrane. Observations of PG/GAG staining patterns resulted in identification of microstructurally based subdivisions of canine, porcine, and ferret LPs.
The sulfated GAG concentration in human LP was similar to that of dermis. In contrast to the interspecies similarity in LP sulfated GAG levels, immunohistochemical analysis indicated notable interspecies differences in specific PG/GAG distributions. Moreover, spatial variations in the presence of several PGs/GAGs were observed--variations that may be integral in maintaining normal LP physiology. Finally, the noted canine, porcine, and ferret LP subdivisions may yield insight into the adaptation of LP microstructure to the phonatory needs of each species.
我们研究了人类、犬类、猪和雪貂的声带膜性层固有层（LP）中的蛋白聚糖（PG）及相关硫酸化糖胺聚糖（GAG）含量。
通过定量相关硫酸化GAG间接评估LP中PG水平，并对特定PG和/或GAG（PGs/GAGs）进行免疫组织化学分析。
硫酸化GAG在人LP中约占（平均±标准误）每毫克组织总蛋白14.7±2.1微克，与犬、猪和雪貂LP中的水平相似（p>.05）。免疫组织化学分析在LP细胞外基质中鉴定出多功能蛋白聚糖、硫酸软骨素4和6硫酸盐以及硫酸乙酰肝素，这些PGs/GAGs以前被认为仅定位于细胞内和基底膜。对PG/GAG染色模式的观察导致识别出犬、猪和雪貂LP基于微观结构的亚区。
人LP中硫酸化GAG浓度与真皮相似。与LP硫酸化GAG水平的种间相似性相反，免疫组织化学分析表明特定PG/GAG分布存在显著种间差异。此外，观察到几种PGs/GAGs存在空间变化，这些变化可能对维持LP正常生理功能至关重要。最后，所指出的犬、猪和雪貂LP亚区可能有助于深入了解LP微观结构对每个物种发声需求的适应性。
