邻苯二甲酸异构体的胶束电动毛细管色谱分析

Micellar electrokinetic capillary chromatography analysis of phthalic acid isomers.

作者信息

Rigout Muriel L A, Lewis David M, Broadbent Peter J

机构信息

Colour and Polymer Chemistry Department, University of Leeds, Leeds, West Yorkshire, UK.

出版信息

J Capill Electrophor Microchip Technol. 2005;9(3-4):57-64.

PMID:16042126

Abstract

A capillary electrophoretic method for the analysis and separation of ortho- and para-phthalic acid isomers was developed. The best separation was achieved using micellar electrokinetic capillary chromatography (MECC) performed in a 110 mM borate buffer pH 8.3 also containing 40 mM sodium dodecyl sulfate (SDS) and 20% methanol. The effect of the addition of methanol, acetonitrile, and beta-cyclodextrin on the separation of the isomers was investigated. The buffer system developed was shown to provide a dependable method of analysis from which the concentration of each analyte could be reliably determined.

摘要

开发了一种用于分析和分离邻苯二甲酸和对苯二甲酸异构体的毛细管电泳方法。使用胶束电动毛细管色谱法（MECC）在pH 8.3的110 mM硼酸盐缓冲液中进行分离，该缓冲液还含有40 mM十二烷基硫酸钠（SDS）和20%甲醇，可实现最佳分离效果。研究了添加甲醇、乙腈和β-环糊精对异构体分离的影响。所开发的缓冲系统被证明提供了一种可靠的分析方法，据此可以可靠地测定每种分析物的浓度。