Gan Xiao-Xian, Zeng Xiao-Peng, Wang Yue, Ding Jian-Zu, Shen Hui-Ying, Shen Li-Ying, Fan Jin-Jiang
Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2005 Apr 30;23(2):97-9, 105.
To investigate the protective immunity against Schistosoma japonicum in mice immunized with recombinant specific very low density lipoprotein binding protein (SVLBP) and its potential as vaccine candidate.
Recombinant SVLBP antigen was over-expressed under IPTG induction and purified by Ni-NTA affinity chromatography. C57BL/6 mice were immunized three times with purified reSVLBP complexed with Freund's adjuvant, at biweekly intervals. Then 35+/-1 cercariae of S. japonicum were given to each mouse by abdominal skin 10 days after the 3rd immunization. 45 days later, all mice were sacrificed to collect adult worms and count liver eggs. serum samples were collected before immunization and after challenge respectively, and were probed the antigen-specific antibodies using a panel of ELISAs.
The worm burden and the egg deposition in liver tissue were reduced by 33.4% and 47.6% respectively in the immunized group, in comparison with the adjuvant control group (P<0.05). Higher titer (>1:6 400) of total IgG was observed after challenge infection. The vaccinated mice developed significantly higher levels of IgG2a, IgG2b, IgG1 than those of control mice.
The recombinant tegumental SVLBP antigen could induce partial protection against S. japonicum infection. These data demonstrate the potential of SVLBP as a schistosome vaccine candidate.
研究用重组特异性极低密度脂蛋白结合蛋白（SVLBP）免疫的小鼠对日本血吸虫的保护性免疫及其作为候选疫苗的潜力。
重组SVLBP抗原在IPTG诱导下过量表达，并通过Ni-NTA亲和层析纯化。C57BL/6小鼠每隔一周用与弗氏佐剂复合的纯化重组SVLBP免疫三次。在第三次免疫后10天，通过腹部皮肤给每只小鼠接种35±1条日本血吸虫尾蚴。45天后，处死所有小鼠以收集成虫并计数肝内虫卵。分别在免疫前和攻击后收集血清样本，并使用一组ELISA检测抗原特异性抗体。
与佐剂对照组相比，免疫组的虫负荷和肝组织内虫卵沉积分别减少了33.4%和47.6%（P<0.05）。攻击感染后观察到总IgG滴度较高（>1:6400）。接种疫苗的小鼠产生的IgG2a、IgG2b、IgG1水平明显高于对照小鼠。
重组体表SVLBP抗原可诱导对日本血吸虫感染的部分保护作用。这些数据证明了SVLBP作为血吸虫候选疫苗的潜力。
