Abdelrahman Yasser M, Belland Robert J
Department of Molecular Sciences, University of Tennessee Health Sciences Center, 858 Madison Avenue, Memphis, TN 38163, USA.
FEMS Microbiol Rev. 2005 Nov;29(5):949-59. doi: 10.1016/j.femsre.2005.03.002.
Intracellular parasitism by bacterial pathogens is a complex, multi-factorial process that has been exploited successfully by a wide variety of organisms. Members of the Order Chlamydiales are obligate intracellular bacteria that are transmitted as metabolically inactive particles and must differentiate, replicate, and re-differentiate within the host cell to carry out their life cycle. Understanding the developmental cycle has been greatly advanced by the availability of complete genome sequences, DNA microarrays, and advanced cell biology techniques. Measuring transcriptional changes throughout the cycle has allowed investigators to determine the nature of the temporal gene expression changes required for bacterial growth and development.
细菌病原体的细胞内寄生是一个复杂的多因素过程，已被多种生物体成功利用。衣原体目成员是专性细胞内细菌，以代谢无活性的颗粒形式传播，必须在宿主细胞内进行分化、复制和再分化才能完成其生命周期。完整基因组序列、DNA微阵列和先进细胞生物学技术的出现极大地推动了对发育周期的理解。测量整个周期中的转录变化使研究人员能够确定细菌生长和发育所需的时间基因表达变化的性质。
