Schimek Christine, Petzold Annett, Schultze Kornelia, Wetzel Jana, Wolschendorf Frank, Burmester Anke, Wöstemeyer Johannes
Department of General Microbiology and Microbial Genetics, Institute of Microbiology, Friedrich-Schiller-University Jena, Neugasse 24, D-07743 Jena, Germany.
Fungal Genet Biol. 2005 Sep;42(9):804-12. doi: 10.1016/j.fgb.2005.05.003.
4-Dihydromethyltrisporate dehydrogenase (TDH) converts the (+) mating type sex pheromone 4-dihydromethyltrisporate into methyltrisporate. In Mucor mucedo, this conversion is required only in the (-) mating type. Expression of the TDH encoding TSP1 gene was analyzed qualitatively using reverse-transcribed PCR. TSP1 is constitutively transcribed in the (+) and in the (-) mating type, irrespective of the mating situation. By immunodetection, the translation product is also formed constitutively. In contrast to gene expression, TDH enzyme activity depends on the sexual status of the mycelium. Activity is restricted to the sexually stimulated (-) mating type. Non-stimulated (-), as well as stimulated and non-stimulated (+) mycelia exhibit no activity and do not influence activity in stimulated (-) mycelia. Time course analysis shows strongly increased enzyme activity at 80 min after stimulation. Low activity exists from the onset of stimulation, indicating that additional regulation mechanisms are involved in TDH function.
4-二氢甲基三孢酸脱氢酶（TDH）将（+）交配型性信息素4-二氢甲基三孢酸转化为甲基三孢酸。在总状毛霉中，这种转化仅在（-）交配型中需要。使用逆转录PCR对编码TSP1基因的TDH进行了定性分析。TSP1在（+）和（-）交配型中均持续转录，与交配情况无关。通过免疫检测，翻译产物也持续形成。与基因表达相反，TDH酶活性取决于菌丝体的性状态。活性仅限于受性刺激的（-）交配型。未受刺激的（-）以及受刺激和未受刺激的（+）菌丝体均无活性，且不影响受刺激的（-）菌丝体中的活性。时间进程分析表明，刺激后80分钟酶活性大幅增加。从刺激开始就存在低活性，这表明TDH功能还涉及其他调节机制。
