Hudecz Ferenc
Department of Organic Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, Budapest, Hungary.
Methods Mol Biol. 2005;298:209-23. doi: 10.1385/1-59259-877-3:209.
Bioconjugates play an important role in several fields of biomolecular and biomedicinal sciences. Protein/polypeptide-based conjugates with covalently attached epitope peptides are considered as potential synthetic vaccine candidates and/or target antigens in affinity-based bioassays. This chapter describes the synthesis of two- and three-component bioconjugates using water-soluble branched chain polymeric polypeptides with multiple amino and/or carboxyl groups as macromolecular partners and oligopeptides as epitopes with small molecular mass. The synthetic procedures outline three major strategies for the incorporation of multiple copies of uniformly oriented peptide epitopes. In the first example, chloroacetylated polypeptide is conjugated with SH-peptide to form a thioether linkage. Second, two independent oligopeptides are introduced into a macromolecule by amide and disulfide bonds, respectively. In the third example, a new procedure is reported for the formation of disulfide bridges by the use of Npys-modified polypeptide and SH-peptide.
生物共轭物在生物分子和生物医学科学的多个领域发挥着重要作用。具有共价连接表位肽的基于蛋白质/多肽的共轭物被认为是潜在的合成疫苗候选物和/或基于亲和力的生物测定中的靶抗原。本章描述了使用具有多个氨基和/或羧基的水溶性支链聚合多肽作为大分子伙伴以及低分子量寡肽作为表位来合成二元和三元生物共轭物。合成程序概述了掺入多个均匀取向的肽表位的三种主要策略。在第一个例子中，氯乙酰化多肽与SH-肽共轭形成硫醚键。其次，通过酰胺键和二硫键分别将两个独立的寡肽引入大分子中。在第三个例子中，报道了一种使用Npys修饰的多肽和SH-肽形成二硫键的新方法。
