He Fang, Wu Li-Xiang, Liu Fa-Yi, Cao Li, Yang Li-Juan, Zhou Xuan, Zhao Yan
Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005 Jun;30(3):266-9.
To explore the effect of hepatocyte growth factor (HGF) on oxygen-glucose deprived injury and apoptosis of astrocytes.
The injury of primary cultured rat cerebral cortical astrocytes was induced by oxygen-glucose deprivation. Astrocytes were treated with HGF at various final concentrations of 20 - 100 ng/mL. The cell damage and viability were evaluated by the lactate dehydrogenase (LDH) released rate and the 3- (4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) conversion method. Detection of apoptotic cells was determined by the flow cytometry, and the ultrastructure was observed by the transmission electron microscope.
Oxygen-glucose deprivation increased the LDH release rate, decreased the cell viability and increased the number of apoptotic astrocytes. While exposed to HGF at the same condition, the LDH release rate decreased, the cell viability increased, and the percentage of apoptotic cells decreased (P <0.05). The maximum protective effect of HGF was observed at 60 ng/mL.
HGF can protect cultured astrocytes from oxygen-glucose deprived injury, and attenuate the apoptosis of astrocytes in a dose-dependent manner.
探讨肝细胞生长因子（HGF）对氧糖剥夺损伤及星形胶质细胞凋亡的影响。
采用氧糖剥夺法诱导原代培养的大鼠大脑皮质星形胶质细胞损伤。用终浓度为20 - 100 ng/mL的HGF处理星形胶质细胞。通过乳酸脱氢酶（LDH）释放率和3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）转化法评估细胞损伤和活力。通过流式细胞术检测凋亡细胞，并用透射电子显微镜观察超微结构。
氧糖剥夺增加了LDH释放率，降低了细胞活力并增加了凋亡星形胶质细胞的数量。在相同条件下暴露于HGF时，LDH释放率降低，细胞活力增加，凋亡细胞百分比降低（P <0.05）。在60 ng/mL时观察到HGF的最大保护作用。
HGF可保护培养的星形胶质细胞免受氧糖剥夺损伤，并以剂量依赖方式减轻星形胶质细胞的凋亡。
