Yuan Lin-Bo, He Qun
Research Laboratory of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005 Jun;30(3):288-91.
To determine the differentiation-inducing effects of perphenazine on K562 leukemia cells.
Differentiation-Inducing effects of a phenothiazine perphenazine were evaluated by proliferation, morphology and function of K562 cells. We evaluated the effects of perphenazine on K562 cells proliferation by cellular enumeration in liquid culture assay, MTT assay and clony formation assay, the morphology by Wight-Gimesa staining, and the function by detecting CD71 through flow cytometry.
Perphenazine enhanced the expression of CD71 on K562 cells and increased Hb content in K562 cells, while inhibited the proliferation of K562 cells. K562 cells showed differentiation morphology after the drug treatment.
Perphenazine possessed differentiation-inducing effects on K562 cells.
确定奋乃静对K562白血病细胞的诱导分化作用。
通过K562细胞的增殖、形态和功能来评估吩噻嗪类药物奋乃静的诱导分化作用。我们通过液体培养试验中的细胞计数、MTT试验和克隆形成试验评估奋乃静对K562细胞增殖的影响，通过瑞氏-吉姆萨染色观察形态，通过流式细胞术检测CD71来评估功能。
奋乃静增强了K562细胞上CD71的表达，增加了K562细胞中的血红蛋白含量，同时抑制了K562细胞的增殖。药物处理后K562细胞呈现出分化形态。
奋乃静对K562细胞具有诱导分化作用。
