Vocks M, Adam C, Lesjean B, Gnirss R, Kraume M
Technische Universität Berlin, Institut für Verfahrenstechnik, Ackerstr. 71-76, ACK 7, D-13355 Berlin, Germany.
Water Res. 2005 Sep;39(14):3360-8. doi: 10.1016/j.watres.2005.05.049.
This study investigates a post-denitrification process without the addition of an external carbon source combined with an enhanced biological phosphorus removal (EBPR) in a membrane bioreactor (MBR). Three trial plants, with two different process configurations, were operated on two different sites, and a variety of accompanying batch tests were conducted. It was shown that even without dosing of an external carbon source, denitrification rates (DNR) much above endogenous rates could be obtained in post-denitrification systems. Furthermore, the anaerobic reactor located ahead of the process had a positive impact on the DNR. Given these surprising results, the project team decided to identify the carbon source used by the microorganisms in the post-denitrification process. Batch tests could demonstrate that lysis products do not play a major role as a C-source for post-denitrification. The following hypothesis was proposed to explain the observations: the glycogen, internally stored by the substrate accumulating bacteria, if anaerobic conditions are followed by aerobic conditions could act as carbon source for denitrification in post-denitrification system. First exploratory batch tests, where the glycogen evolution was monitored, corroborate this assumption.
本研究调查了膜生物反应器（MBR）中不添加外部碳源的后置反硝化过程与强化生物除磷（EBPR）相结合的情况。在两个不同地点运行了三个试验装置，采用两种不同的工艺配置，并进行了各种配套的批次试验。结果表明，即使不投加外部碳源，后置反硝化系统也能获得远高于内源速率的反硝化速率（DNR）。此外，位于该工艺前端的厌氧反应器对DNR有积极影响。鉴于这些惊人的结果，项目团队决定确定后置反硝化过程中微生物所使用的碳源。批次试验表明，细胞裂解产物在后置反硝化中并非主要的碳源。为解释这些观察结果，提出了以下假设：如果厌氧条件之后是好氧条件，底物积累细菌内部储存的糖原可作为后置反硝化系统中反硝化的碳源。首次监测糖原演变的探索性批次试验证实了这一假设。
