Liu Yindong, Su Xiaomei, Lu Lian, Ding Linxian, Shen Chaofeng
Department of Environmental Engineering, Zhejiang University, Yuhangtang Road 866#, Hangzhou, 310058, People's Republic of China.
College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, 321004, China.
Environ Sci Pollut Res Int. 2016 Mar;23(5):4498-508. doi: 10.1007/s11356-015-5603-3. Epub 2015 Oct 29.
A culture supernatant from Micrococcus luteus containing resuscitation-promoting factor (SRpf) was used to enhance the biological nutrient removal of potentially functional bacteria. The obtained results suggest that SRpf accelerated the start-up process and significantly enhanced the biological nutrient removal in sequencing batch reactor (SBR). PO4 (3-)-P removal efficiency increased by over 12 % and total nitrogen removal efficiency increased by over 8 % in treatment reactor acclimated by SRpf compared with those without SRpf addition. The Illumina high-throughput sequencing analysis showed that SRpf played an essential role in shifts in the composition and diversity of bacterial community. The phyla of Proteobacteria and Actinobacteria, which were closely related to biological nutrient removal, were greatly abundant after SRpf addition. This study demonstrates that SRpf acclimation or addition might hold great potential as an efficient and cost-effective alternative for wastewater treatment plants (WWTPs) to meet more stringent operation conditions and legislations.
使用含有复苏促进因子（SRpf）的藤黄微球菌培养上清液来提高潜在功能细菌的生物营养物去除能力。所得结果表明，SRpf加速了启动过程，并显著提高了序批式反应器（SBR）中的生物营养物去除效果。与未添加SRpf的处理反应器相比，经SRpf驯化的处理反应器中PO4(3 -)-P去除效率提高了12%以上，总氮去除效率提高了8%以上。Illumina高通量测序分析表明，SRpf在细菌群落组成和多样性的变化中起着至关重要的作用。添加SRpf后，与生物营养物去除密切相关的变形菌门和放线菌门大量增加。本研究表明，对于污水处理厂（WWTPs）而言，驯化或添加SRpf可能具有巨大潜力，是一种高效且经济高效的替代方法，可满足更严格的运行条件和法规要求。
