采用胶束液相色谱法测定阿米替林和去甲替林血清浓度。
Amitriptyline and nortriptyline serum determination by micellar liquid chromatography.
作者信息
Bose Devasish, Durgbanshi Abhilasha, Martinavarro-Domínguez Adrià, Capella-Peiró Maria-Elisa, Carda-Broch Samuel, Esteve-Romero Josep, Gil-Agustí Mayte
机构信息
Area de Química Analítica, Universitat Jaume I, Campus Riu Sec, Av. de Vicent Sos Baynat, s/n, 12071 Castelló, Spain.
出版信息
J Pharmacol Toxicol Methods. 2005 Nov-Dec;52(3):323-9. doi: 10.1016/j.vascn.2005.04.015. Epub 2005 Jul 20.
INTRODUCTION
Amitriptyline and nortriptyline are tricyclic antidepressants which act by enhancing the actions of norepinephrine and serotonin caused by blocking the re-uptake of various neurotransmitters at the neuronal membrane. A micellar liquid chromatographic procedure was developed to determine these drugs in serum samples for use in clinical monitoring.
METHODS
The chromatographic determination of these highly hydrophobic substances was carried out using a 0.15 M SDS-6% (v/v) pentanol buffered at pH 7, in a C18 column, and electrochemical detection at 650 mV. The flow-rate was 1.5 mL/min. The analysis time was 14 min.
RESULTS
The limits of detection (ng/mL) in serum were 0.25 and 0.31 for amitriptyline and nortriptyline, respectively. Repeatability and intermediate precision were evaluated at three different concentrations in serum samples.
DISCUSSION
Untreated serum samples were injected directly into the HPLC system after filtration, leading to be a simple procedure that can be applied in routine analyses for Therapeutic Drug Monitoring.
引言
阿米替林和去甲替林是三环类抗抑郁药，其作用机制是通过阻断神经细胞膜上各种神经递质的再摄取来增强去甲肾上腺素和5-羟色胺的作用。开发了一种胶束液相色谱法来测定血清样品中的这些药物，用于临床监测。
方法
使用在pH 7下缓冲的0.15 M SDS - 6%（v/v）戊醇，在C18柱中对这些高度疏水的物质进行色谱测定，并在650 mV下进行电化学检测。流速为1.5 mL/min。分析时间为14分钟。
结果
血清中阿米替林和去甲替林的检测限（ng/mL）分别为0.25和0.31。在血清样品中的三种不同浓度下评估了重复性和中间精密度。
讨论
未处理的血清样品经过过滤后直接注入高效液相色谱系统，这是一个简单的程序，可应用于治疗药物监测的常规分析。
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