Exarchou Vassiliki, Krucker Manfred, van Beek Teris A, Vervoort Jacques, Gerothanassis Ioannis P, Albert Klaus
NMR Center, Department of Chemistry, University of Ioannina, Greece.
Magn Reson Chem. 2005 Sep;43(9):681-7. doi: 10.1002/mrc.1632.
An overview of recent advances in nuclear magnetic resonance (NMR) coupled with separation technologies and their application in natural product analysis is given and discussed. The different modes of LC-NMR operation are described, as well as how technical improvements assist in establishing LC-NMR as an important tool in the analysis of plant-derived compounds. On-flow, stopped-flow and loop-storage procedures are mentioned, together with the new LC-SPE-NMR configuration. The implementation of mass spectrometry in LC-NMR is also useful on account of the molecular weight and fragmentation information that it provides, especially when new plant species are studied. Cryogenic technology and capillary LC-NMR are the other important recent developments. Since the plant kingdom is endless in producing potential drug candidates, development and optimization of LC-NMR techniques convert the study of natural products to a less-time-consuming task, speeding up identification.
本文介绍并讨论了核磁共振（NMR）与分离技术相结合的最新进展及其在天然产物分析中的应用。描述了液相色谱 - 核磁共振（LC - NMR）的不同操作模式，以及技术改进如何有助于将LC - NMR确立为分析植物源化合物的重要工具。文中提到了在线、停流和环式储存程序，以及新的LC - SPE - NMR配置。由于液相色谱 - 质谱联用（LC - NMR）中的质谱分析法提供了分子量和碎片信息，特别是在研究新植物物种时，其应用也很有用。低温技术和毛细管LC - NMR是近期的其他重要进展。由于植物界在产生潜在药物候选物方面无穷无尽，LC - NMR技术的开发和优化将天然产物的研究转变为一项耗时较少的任务，加快了鉴定速度。
