红鳍东方鲀线粒体腺嘌呤核苷酸转运体三种同工型的鉴定。

Identification of three isoforms for mitochondrial adenine nucleotide translocator in the pufferfish Takifugu rubripes.

作者信息

Itoi Shiro, Misaki Ryohei, Hirayama Makoto, Nakaniwa Makiko, Liang Chun-Shi, Kondo Hidehiro, Watabe Shugo

机构信息

Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.

出版信息

Mitochondrion. 2005 Jun;5(3):162-72. doi: 10.1016/j.mito.2005.01.003.

DOI:10.1016/j.mito.2005.01.003

PMID:16050982

Abstract

Three adenine nucleotide translocator (ANT) genes were identified through in silico data mining of the Fugu genome database along with isolation of their corresponding cDNAs in vivo from the pufferfish (Takifugu rubripes). As a result of phylogenetic analysis, the ANT gene on scaffold_254 corresponded to mammalian ANT1, whereas both of those on scaffold_6 and scaffold_598 to mammalian ANT3. The ANT gene encoded by scaffold_6 was expressed ubiquitously in various tissues, whereas the ANT genes encoded by scaffold_254 and scaffold_598 were predominantly expressed in skeletal muscle and heart, respectively.

摘要

通过对河豚基因组数据库进行电子数据挖掘，并从河豚（红鳍东方鲀）体内分离出相应的cDNA，鉴定出了三个腺嘌呤核苷酸转运体（ANT）基因。系统发育分析结果表明，scaffold_254上的ANT基因对应于哺乳动物的ANT1，而scaffold_6和scaffold_598上的两个基因对应于哺乳动物的ANT3。scaffold_6编码的ANT基因在各种组织中普遍表达，而scaffold_254和scaffold_598编码的ANT基因分别主要在骨骼肌和心脏中表达。