通过全自动分子检测法快速诊断腺病毒性角结膜炎
Rapid diagnosis of adenoviral keratoconjunctivitis by a fully automated molecular assay.
作者信息
Koidl Christoph, Bozic Michael, Mossböck Georg, Mühlbauer Gerhard, Berg Jörg, Stöcher Markus, Dehnhardt Jasmin, Marth Egon, Kessler Harald H
机构信息
Institute of Hygiene, Medical University of Graz, Graz, Austria.
出版信息
Ophthalmology. 2005 Sep;112(9):1521-8. doi: 10.1016/j.ophtha.2005.04.011.
OBJECTIVE
To establish and evaluate a new test system for rapid detection and diagnosis of adenoviral keratoconjunctivitis.
DESIGN
After establishment of the molecular assay, 52 conjunctival smears were studied.
PARTICIPANTS
Samples were derived from patients with a clinical presentation compatible with keratoconjunctivitis.
METHODS
A molecular assay for detection of human adenovirus (HAdV) based on automated nucleic acid extraction and real time polymerase chain reaction was established and evaluated. The new assay included a heterologous internal control.
MAIN OUTCOME MEASURES
Statement about the presence or absence of adenoviral DNA in the specimen.
RESULTS
The amplification efficiency was found to be 100%. The detection limit was calculated to be 116 copies per LightCycler capillary. When clinical specimens were tested, 15 of 52 conjunctival smears were found to be positive for HAdV DNA. The internal control was detected in all samples.
CONCLUSIONS
The new molecular assay proved to be suitable for rapid diagnosis of adenoviral keratoconjunctivitis in the routine diagnostic laboratory.
目的
建立并评估一种用于快速检测和诊断腺病毒性角结膜炎的新检测系统。
设计
建立分子检测方法后，对52份结膜涂片进行研究。
参与者
样本取自临床表现符合角结膜炎的患者。
方法
建立并评估一种基于自动核酸提取和实时聚合酶链反应的检测人腺病毒（HAdV）的分子检测方法。新检测方法包括一个异源内部对照。
主要观察指标
关于样本中腺病毒DNA存在与否的声明。
结果
扩增效率为100%。检测限经计算为每个LightCycler毛细管116个拷贝。对临床样本进行检测时，发现52份结膜涂片中15份HAdV DNA呈阳性。所有样本中均检测到内部对照。
结论
新的分子检测方法被证明适用于常规诊断实验室快速诊断腺病毒性角结膜炎。
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