Dincić Dragan, Zivković Maja, Stanković Aleksandra, Djurić Tamara, Vujanić Svetlana, Gligić Branko, Alavantić Dragan
Clinic for Urgent Internal Medicine, Military Medical Academy, Belgrade, Serbia and Montenegro.
Wien Klin Wochenschr. 2005 Jun;117(11-12):406-11. doi: 10.1007/s00508-005-0358-3.
Lipoprotein(a) (Lp(a)) concentration is determined primarily by the apolipoprotein(a) (apo(a)) gene. The pentanucleotide (TTTTA)n repeat and G/A-914 polymorphisms are in the 5' promoter region of the apo(a) gene. To elucidate whether these polymorphisms affect Lp(a) levels, a total of 211 Serbian adults were investigated.
One hundred and eleven patients with ischemic heart disease and 100 healthy controls were genotyped and Lp(a) levels determined.
Lp(a) concentrations differed according to the (TTTTA)n genotypes: among those having at least one allele 8, patients had significantly higher Lp(a) values than controls. A decreasing trend of Lp(a) values was associated with the -914A allele in controls but the opposite was true in patients. Patients with genotype TTTTA allele 8/AA-914 had significantly higher Lp(a) values than those without allele 8/AA (p < 0.05). The >8>8/GG genotype was not detected. Significant linkage disequilibrium between (TTTTA)n and G/A-914 polymorphism (p < 0.001) was found. In multivariate regression analysis, the G/A-914 polymorphism significantly (p < 0.05) affected Lp(a) levels in patients, after taking into account the (TTTTA)n polymorphism.
These results indicate that (TTTTA)n and G/A-914 polymorphisms affect Lp(a) levels in ischemic heart disease as a consequence of the linkage disequlibrium.
脂蛋白(a)（Lp(a)）浓度主要由载脂蛋白(a)（apo(a)）基因决定。五核苷酸(TTTTA)n重复序列和G/A - 914多态性位于apo(a)基因的5'启动子区域。为阐明这些多态性是否影响Lp(a)水平，共对211名塞尔维亚成年人进行了研究。
对111例缺血性心脏病患者和100名健康对照者进行基因分型并测定Lp(a)水平。
Lp(a)浓度根据(TTTTA)n基因型不同而有所差异：在至少有一个8等位基因的人群中，患者的Lp(a)值显著高于对照者。在对照者中，Lp(a)值的下降趋势与 - 914A等位基因相关，但在患者中情况相反。基因型为TTTTA等位基因8/AA - 914的患者的Lp(a)值显著高于无等位基因8/AA的患者（p < 0.05）。未检测到>8>8/GG基因型。发现(TTTTA)n与G/A - 914多态性之间存在显著的连锁不平衡（p < 0.001）。在多变量回归分析中，在考虑(TTTTA)n多态性后，G/A - 914多态性对患者的Lp(a)水平有显著影响（p < 0.05）。
这些结果表明，由于连锁不平衡，(TTTTA)n和G/A - 914多态性影响缺血性心脏病患者的Lp(a)水平。
