Carroll Thomas J, Park Joo-Seop, Hayashi Shigemi, Majumdar Arindam, McMahon Andrew P
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
Dev Cell. 2005 Aug;9(2):283-92. doi: 10.1016/j.devcel.2005.05.016.
The vertebrate urogenital system forms due to inductive interactions between the Wolffian duct, its derivative the ureteric bud, and their adjacent mesenchymes. These establish epithelial primordia within the mesonephric (embryonic) and metanephric (adult) kidneys and the Müllerian duct, the anlage of much of the female reproductive tract. We show that Wnt9b is expressed in the inductive epithelia and is essential for the development of mesonephric and metanephric tubules and caudal extension of the Müllerian duct. Wnt9b is required for the earliest inductive response in metanephric mesenchyme. Further, Wnt9b-expressing cells can functionally substitute for the ureteric bud in these interactions. Wnt9b acts upstream of another Wnt, Wnt4, in this process, and our data implicate canonical Wnt signaling as one of the major pathways in the organization of the mammalian urogenital system. Together these findings suggest that Wnt9b is a common organizing signal regulating diverse components of the mammalian urogenital system.
脊椎动物的泌尿生殖系统是由沃尔夫管、其衍生物输尿管芽及其相邻间充质之间的诱导相互作用形成的。这些相互作用在中肾（胚胎期）和后肾（成体）肾脏以及缪勒管（女性大部分生殖道的原基）内建立上皮原基。我们发现，Wnt9b在诱导上皮中表达，对中肾小管和后肾小管的发育以及缪勒管的尾端延伸至关重要。Wnt9b是后肾间充质最早诱导反应所必需的。此外，表达Wnt9b的细胞在这些相互作用中可在功能上替代输尿管芽。在此过程中，Wnt9b在另一种Wnt即Wnt4的上游起作用，我们的数据表明经典Wnt信号通路是哺乳动物泌尿生殖系统组织中的主要途径之一。这些发现共同表明，Wnt9b是调节哺乳动物泌尿生殖系统不同组成部分的共同组织信号。
