来自两种巴西大西洋虾类，即南美白对虾和施氏滨对虾的对虾抗菌肽的分子特征分析。
Molecular characterization of penaeidins from two Atlantic Brazilian shrimp species, Farfantepenaeus paulensis and Litopenaeus schmitti.
作者信息
Barracco Margherita Anna, de Lorgeril Julien, Gueguen Yannick, Bachère Evelyne
机构信息
Departamento de Biologia Celular, Embriologia e Genética, Universidade Federal de Santa Catarina, Florianópolis/SC, Brazil.
出版信息
FEMS Microbiol Lett. 2005 Sep 1;250(1):117-20. doi: 10.1016/j.femsle.2005.06.055.
We report here the molecular cloning of new members of the penaeidin family from two Atlantic penaeids from Brazil, Litopenaeus schmitti and Farfantepenaeus paulensis. The presence of penaeidins in the granular hemocytes of both shrimps was first evidenced by immunofluorescence, using polyclonal antibodies raised against L. vannamei penaeidin Litvan PEN3-1. cDNAs from the hemocytes of both Brazilian species were obtained by reverse transcription and the sequences encoding penaeidins were amplified by PCR, using primers based on penaeidin consensus sequences. Five penaeidin clones were obtained. According to the international penaeidin classification (PenBase, http://www.penbase.immunaqua.com), the deduced amino acid sequences of two clones from L. schmitti and two from F. paulensis belong to the PEN2 subgroup and one clone from L. schmitti to the PEN4 subgroup of penaeidins. Surprisingly, no penaeidin from the PEN3 subgroup was obtained in both shrimp species, even though this subgroup appears to be the most commonly expressed in the hemocytes of penaeids.
我们在此报告从巴西的两种大西洋对虾，即施氏滨对虾（Litopenaeus schmitti）和保罗氏对虾（Farfantepenaeus paulensis）中克隆对虾抗菌肽家族新成员的情况。利用针对凡纳滨对虾抗菌肽Litvan PEN3 - 1制备的多克隆抗体，通过免疫荧光首次证实了这两种对虾的颗粒血细胞中存在对虾抗菌肽。通过逆转录获得这两种巴西对虾血细胞的cDNA，并使用基于对虾抗菌肽共有序列的引物，通过聚合酶链反应（PCR）扩增编码对虾抗菌肽的序列。获得了5个对虾抗菌肽克隆。根据国际对虾抗菌肽分类（PenBase，http://www.penbase.immunaqua.com），施氏滨对虾的两个克隆和保罗氏对虾的两个克隆推导的氨基酸序列属于对虾抗菌肽的PEN2亚组，施氏滨对虾的一个克隆属于对虾抗菌肽的PEN4亚组。令人惊讶的是，在这两种对虾中均未获得PEN3亚组的对虾抗菌肽，尽管该亚组似乎是在对虾血细胞中最常表达的。
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