Yavuz Erdem, Morawski Krzysztof, Telischi Fred F, Ozdamar Ozcan, Delgado Rafael E, Manns Fabrice, Parel Jean-Marie
Department of Biomedical Engineering, University of Miami, Coral Gables, FL 33124, USA.
J Neurosci Methods. 2005 Aug 30;147(1):55-64. doi: 10.1016/j.jneumeth.2005.03.004. Epub 2005 Apr 25.
In this study, a new monitoring system is developed to measure cochlear blood flow (CBF) and electrocochleography (ECochG) during transient ischemic episodes of the cochlea. A newly designed otic probe was used for the simultaneous recordings of laser-Doppler CBF and ECochG directly from the round window (RW). The probe enabled the recording of high amplitude compound action potentials (CAP) and cochlear microphonics (CM) with few averages. Experiments were conducted on rabbits to generate episodes of cochlear ischemia by using timed compressions of the internal auditory artery (IAA). The computer monitoring system extracted and measured CAP and CM components from ECochG in real-time. Results indicate that CM and CAP generally followed CBF during compressions and releases of IAA. Both CBF values and CAP amplitudes showed an overshoot following the reperfusion. CAP amplitude measures were found to be very sensitive to ischemia showing very rapid amplitude, latency and morphological changes. CM amplitude decreased more slowly than the CAP and CBF. Simultaneous recordings of CBF and ECochG using the otic probe provide a valuable neuromonitoring tool to investigate the dynamic behavior of the cochlea during ischemia.
在本研究中，开发了一种新的监测系统，用于在耳蜗短暂缺血发作期间测量耳蜗血流量（CBF）和耳蜗电图（ECochG）。一种新设计的耳探头用于直接从圆窗（RW）同时记录激光多普勒CBF和ECochG。该探头能够以较少的平均次数记录高振幅复合动作电位（CAP）和耳蜗微音电位（CM）。通过定时压迫家兔的内听动脉（IAA）来诱发耳蜗缺血发作并进行实验。计算机监测系统实时从ECochG中提取并测量CAP和CM成分。结果表明，在IAA压迫和松开期间，CM和CAP通常随CBF变化。再灌注后，CBF值和CAP振幅均出现过冲。发现CAP振幅测量对缺血非常敏感，显示出非常快速的振幅、潜伏期和形态学变化。CM振幅下降比CAP和CBF更慢。使用耳探头同时记录CBF和ECochG提供了一种有价值的神经监测工具，用于研究缺血期间耳蜗的动态行为。
