Möstl Erich, Rettenbacher Sophie, Palme Rupert
Institute of Biochemistry, Department of Natural Sciences, University of Veterinary Medicine, Vienna, Veterinärplatz 1, A-1210 Vienna, Austria.
Ann N Y Acad Sci. 2005 Jun;1046:17-34. doi: 10.1196/annals.1343.004.
Fecal steroid analyses are becoming increasingly popular among both field and laboratory scientists. The benefits associated with sampling procedures that do not require restraint, anesthesia, and blood collection include less risk to subject and investigator, as well as the potential to obtain endocrine profiles that are not influenced by the sampling procedure itself. In the feces, a species-specific pattern of metabolites is present, because glucocorticoids are extensively metabolized. Therefore, selection of adequate extraction procedures and immunoassays for measuring the relevant metabolites is a serious issue. In this review, emphasis is placed on the establishment and analytical validation of methods to measure glucocorticoid metabolites for a noninvasive evaluation of adrenocortical activity in droppings of birds.
粪便类固醇分析在野外和实验室科学家中越来越受欢迎。与无需约束、麻醉和采血的采样程序相关的好处包括对受试者和研究者的风险较小,以及有可能获得不受采样程序本身影响的内分泌概况。由于糖皮质激素会被广泛代谢,粪便中存在特定物种的代谢物模式。因此,选择合适的提取程序和免疫测定法来测量相关代谢物是一个重要问题。在这篇综述中,重点是建立和分析验证用于测量鸟类粪便中糖皮质激素代谢物的方法,以对肾上腺皮质活动进行无创评估。