用于筛选人类免疫缺陷病毒包膜糖蛋白与其细胞受体CD4之间相互作用抑制剂的简易检测方法。
Simple assay to screen for inhibitors of interaction between the human immunodeficiency virus envelope glycoprotein and its cellular receptor, CD4.
作者信息
Chams V, Idziorek T, Maddon P J, Klatzmann D
机构信息
Laboratoire de Biologie et Génétique des Infections Rétrovirales, Groupe Hospitalier Pitié-Salpêtrière, URA Centre National de la Recherche Scientifique 1463, Paris, France.
出版信息
Antimicrob Agents Chemother. 1992 Feb;36(2):267-72. doi: 10.1128/AAC.36.2.267.
Abstract
The binding of the human immunodeficiency virus envelope glycoprotein gp120 to the CD4 molecule is the initial step in the viral replicative cycle. This interaction is therefore an important target for therapeutic intervention for the treatment of human immunodeficiency virus infection. We designed an enzyme-linked immunosorbent assay which detects the interaction between recombinant soluble forms of CD4 and gp160. This assay could be used as an initial screen of libraries of synthetic chemical compounds and natural products.
摘要
人类免疫缺陷病毒包膜糖蛋白gp120与CD4分子的结合是病毒复制周期的起始步骤。因此，这种相互作用是治疗人类免疫缺陷病毒感染进行治疗性干预的重要靶点。我们设计了一种酶联免疫吸附测定法，用于检测重组可溶性形式的CD4和gp160之间的相互作用。该测定法可作为合成化合物库和天然产物库的初步筛选方法。
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