Aghaallaei Narges, Bajoghli Baubak, Walter Ingrid, Czerny Thomas
Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Vienna, Austria.
Dev Dyn. 2005 Sep;234(1):143-50. doi: 10.1002/dvdy.20510.
The highly conserved Groucho/Tle gene family has widespread functions during embryonic development and in adults. For mammalians, four full-length Tle paralogues are known, whereas the whole spectrum of this gene family in fish species has not been analysed yet. Most detailed data exist for medaka, where 3 Tle genes have been described, Tle1, Tle3, and Tle4. We now isolated 3 additional Tle genes from the medaka genome. Sequence analysis identifies these genes as Tle2a, Tle2b, and Tle3b. Database searches of genomic sequences revealed an identical set of Tle paralogues being present in distantly related fish species, indicating duplicated Tle2 and Tle3 genes for the complete teleost lineage. Like the previously analysed medaka Tle genes, the three new genes show a broad expression pattern during embryogenesis. Nevertheless, a detailed comparison of all six Tle genes reveals critical differences in certain aspects of their expression pattern. In particular, we concentrated on the activity of Tle genes during ear development and found Tle2a and Tle2b expressed in this sensory organ.
高度保守的Groucho/Tle基因家族在胚胎发育和成年期具有广泛的功能。对于哺乳动物来说,已知有四个全长的Tle旁系同源物,而鱼类中该基因家族的全貌尚未进行分析。关于青鳉的详细数据最多,已描述了3个Tle基因,即Tle1、Tle3和Tle4。我们现在从青鳉基因组中分离出另外3个Tle基因。序列分析将这些基因鉴定为Tle2a、Tle2b和Tle3b。对基因组序列进行数据库搜索发现,在远缘鱼类物种中存在一组相同的Tle旁系同源物,这表明整个硬骨鱼谱系中存在Tle2和Tle3基因的重复。与之前分析的青鳉Tle基因一样,这三个新基因在胚胎发育过程中表现出广泛的表达模式。然而,对所有六个Tle基因进行详细比较后发现,它们在表达模式的某些方面存在关键差异。特别是,我们重点研究了Tle基因在耳朵发育过程中的活性,发现Tle2a和Tle2b在这个感觉器官中表达。