Kochhar N, Kaul D
Department of Experimental Medicine, P.G.I.M.E.R., Chandigarh, India.
Blood Coagul Fibrinolysis. 1992 Apr;3(2):215-9. doi: 10.1097/00001721-199204000-00012.
Because of the unresolved complex role of membrane cholesterol content in platelet activity, the present study was addressed to understand two specific issues; firstly whether variation in membrane cholesterol content could influence cyclic nucleotide levels within platelets, and secondly whether the membrane cholesterol modulated cyclic nucleotide levels were mediated through phospholipase A2 activation. The results of this in vitro study revealed that membrane cholesterol not only had the inherent capacity to induce changes in the levels of cyclic nucleotides within human platelets but also that this effect was mediated through its ability to modulate phospholipase A2 activity. Based on these results we suggest that membrane cholesterol modulated phospholipase A2 activity may be the molecular link between the membrane cholesterol content and cyclic nucleotides within human platelets.
由于膜胆固醇含量在血小板活性中尚未明确的复杂作用，本研究旨在了解两个具体问题：其一，膜胆固醇含量的变化是否会影响血小板内的环核苷酸水平；其二，膜胆固醇调节的环核苷酸水平是否通过磷脂酶A2的激活介导。这项体外研究的结果表明，膜胆固醇不仅具有诱导人血小板内环核苷酸水平变化的内在能力，而且这种作用是通过其调节磷脂酶A2活性的能力介导的。基于这些结果，我们认为膜胆固醇调节的磷脂酶A2活性可能是人血小板中膜胆固醇含量与环核苷酸之间的分子联系。
