Radiation induced loss of anti-Ig binding ability of lymphocytes.

The effect of irradiation on the anti Ig binding ability of lymphocytes after irradiation was studied. Normal control and irradiated lymphoid cells were treated with rabbit anti mouse IgG fluorescein conjugated serum and the fluorescein positive cells compared. A reduction in the proportion of stained cells in the irradiated lymphocytes was found. It depends on the dose and on the incubation time at 37 degrees C after irradiation. The maximum effect was detected after 10 minutes of incubation, longer incubation is associated with a partial recovery of the stainability of the IgG receptors.