Hagen J W, Semmelink J M, Klein C P, Prahl-Andersen B, Burger E H
Department of Oral Cell Biology, Academic Center for Dentistry Amsterdam, ACTA, Vrije Universiteit, The Netherlands.
J Biomed Mater Res. 1992 Jul;26(7):897-913. doi: 10.1002/jbm.820260706.
Allogeneic demineralized bone (DB) powder was applied to subcutaneous pockets and cranial defects of rats and histological, histomorphometrical, and radiological evaluation was performed one to 52 weeks after implantation. In both type of implants cartilage formation was observed after 1 week between DB particles and in former vascular channels and cervices within the particles. Foci of bone formation were observed after 2 weeks in the center of the implant. Remineralization of DB particles only occurred in close contact with the new bone tissue by which they became incorporated, indicating that remineralization of DB is a continuation of a process which has started in live bone. At all times, the interface between implant and connective tissue of the host consisted of a layer of non-remineralized DB particles embedded in fibrous connective tissue. This rim was thinner at the dura-mater-side in the cranial implants, compared with the skin-side of the same implants as well as all sides of the subcutaneous implants. The rim became thinner with time but never disappeared completely. The outer contour of the mineralized implant sometimes ran right through a DB particle. A critical concentration of bone-inducing agent leaking from DB particles seems to be necessary for bone induction. We suggest that at the interface of the implant and loose connective tissue this concentration is never reached, which results in maintenance of the rim.
将同种异体脱矿骨（DB）粉末应用于大鼠的皮下囊袋和颅骨缺损处，并在植入后1至52周进行组织学、组织形态计量学和放射学评估。在两种类型的植入物中，1周后在DB颗粒之间以及颗粒内先前的血管通道和颈部观察到软骨形成。2周后在植入物中心观察到骨形成灶。DB颗粒的再矿化仅发生在与新骨组织紧密接触的情况下，通过这种接触它们被整合进去，这表明DB的再矿化是一个在活骨中已经开始的过程的延续。在任何时候，植入物与宿主结缔组织之间的界面都由一层嵌入纤维结缔组织中的未再矿化的DB颗粒组成。与同一植入物的皮肤侧以及皮下植入物的所有侧面相比，颅骨植入物硬脑膜侧的这一边缘更薄。随着时间的推移，边缘变薄但从未完全消失。矿化植入物的外轮廓有时会直接穿过一个DB颗粒。从DB颗粒中泄漏的骨诱导剂的临界浓度似乎是骨诱导所必需的。我们认为，在植入物与疏松结缔组织的界面处，这个浓度永远无法达到，这导致了边缘的维持。
