Sánchez Pilar, Torres Jesús M, Ortega Esperanza
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Avda. de Madrid s/n, Granada, Spain.
Neurochem Res. 2005 Apr;30(4):577-81. doi: 10.1007/s11064-005-2692-2.
We studied the expression of type 1 (5alpha-R1) and type 2 (5alpha-R2) 5alpha-reductase isozymes (5alpha-R) and their regulation by dihydrotestosterone (DHT) in the prefrontal cortex of male and female rats during postnatal sexual differentiation of the central nervous system (CNS), using one-step quantitative RT-PCR coupled with laser-induced fluorescence capillary electrophoresis. We found a higher expression of 5alpha-R2, which is considered a masculinizing enzyme, in the female versus male CNS, and observed sexual dimorphism in the regulation of both 5alpha-R isozymes by DHT. These results open up a new research line that could improve understanding of the role of 5alpha-R isozymes in the physiology of the CNS.
我们使用一步定量逆转录聚合酶链反应结合激光诱导荧光毛细管电泳技术，研究了雄性和雌性大鼠在中枢神经系统（CNS）出生后性分化期间，前额叶皮质中1型（5α - 还原酶1，5alpha-R1）和2型（5α - 还原酶2，5alpha-R2）5α - 还原酶同工酶（5α - R）的表达及其受双氢睾酮（DHT）的调节。我们发现，被认为具有男性化作用的5α - R2在雌性CNS中的表达高于雄性，并观察到DHT对两种5α - R同工酶的调节存在性别差异。这些结果开辟了一条新的研究途径，有助于增进对5α - R同工酶在CNS生理学中作用的理解。
