Wang S F, Ridsdill-Smith T J, Ghisalberti E L
Centre for Legumes in Mediterranean Agriculture, Department of Chemistry, University of Western Australia, Nedlands, Western Australia 6907, Australia.
J Agric Food Chem. 2005 Aug 10;53(16):6240-5. doi: 10.1021/jf0502202.
Trifolium glanduliferum Boiss. was resistant to redlegged earth mite, Halotydeus destructor Tucker (Acari: Penthaleidae), feeding compared with Trifolium subterraneum L. Extracts from T. glanduliferum leaves, obtained with dichloromethane and methanol, acted as feeding deterrents to the redlegged earth mite. Bioassay-directed fractionation of the extracts led to the isolation of several metabolites, coumarin, alpha-and beta-ionone, linalool, medicarpin, that were shown to be deterrent in laboratory experiments. The compounds contributing to the defense of T. glanduliferum were different from those influencing mite feeding on T. subterraneum leaves.
腺毛三叶草（Trifolium glanduliferum Boiss.）比地下三叶草（Trifolium subterraneum L.）更抗红腿土螨（Halotydeus destructor Tucker，蜱螨亚纲：戊螨科）的取食。用二氯甲烷和甲醇从腺毛三叶草叶片中提取的提取物对红腿土螨具有取食驱避作用。提取物的生物测定导向分级分离导致分离出几种代谢物，香豆素、α-和β-紫罗兰酮、芳樟醇、苜蓿素，这些代谢物在实验室实验中显示出驱避作用。腺毛三叶草防御作用的化合物与影响螨类取食地下三叶草叶片的化合物不同。
