Khawaled K, Cohen T, Zaritsky A
Department of Life Sciences, Ben-Gurion University of the Negev, Be'er-Sheva, Israel.
J Invertebr Pathol. 1992 Mar;59(2):186-9. doi: 10.1016/0022-2011(92)90031-x.
The larvicidal activity of Bacillus thuringiensis var. israelensis against mosquitoes and the blackfly is included in parasporal crystalline bodies which are produced during sporulation. Following ingestion, the crystals are solubilized in the larval midgut and induce death within a short time; the spores germinate in the dead larvae and complete a growth cycle. The fate of the spores in surviving live larvae was elucidated by using a nonlarvicidal B. thuringiensis var. israelensis mutant. When introduced as the only food source, spores of this mutant support development to the adult stage of newly hatched Aedes aegypti larvae at a rate directly related to spore concentration. The conclusion that spores of B. thuringiensis var. israelensis are digested in the larval gut was substantiated by following the incorporation of [35S]methionine-labeled spores into larval tissues.
苏云金芽孢杆菌以色列变种对蚊子和蚋的杀幼虫活性包含在孢子形成过程中产生的伴孢晶体中。幼虫摄入后，晶体会在幼虫中肠溶解，并在短时间内导致死亡；孢子在死亡的幼虫中萌发并完成生长周期。通过使用一种无杀幼虫活性的苏云金芽孢杆菌以色列变种突变体，阐明了存活的活幼虫中孢子的命运。当作为唯一食物来源引入时，这种突变体的孢子以与孢子浓度直接相关的速率支持新孵化的埃及伊蚊幼虫发育到成虫阶段。通过追踪[35S]甲硫氨酸标记的孢子掺入幼虫组织，证实了苏云金芽孢杆菌以色列变种的孢子在幼虫肠道中被消化的结论。
