Misch D W, Burnside D F, Cecil T L
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
J Invertebr Pathol. 1992 May;59(3):286-9. doi: 10.1016/0022-2011(92)90134-p.
A bioassay system employing acutely toxic concentrations of a spore-crystal mixture of Bacillus thuringiensis subspecies israelensis against fourth instar larvae of Aedes aegypti (L.) is described. Individual larvae are separately exposed to toxin in glass-lined miniature wells or scintillation vials. This method is free from the deaths due to predation among larvae. Such larval deaths are commonly encountered in bioassay groups of 25 larvae as currently specified in the World Health Organization guidelines. Our method offers shortened testing time, increased accuracy, and improved statistical precision.
描述了一种生物测定系统，该系统使用苏云金芽孢杆菌以色列亚种的芽孢-晶体混合物的急性毒性浓度来作用于埃及伊蚊（L.）的四龄幼虫。将单个幼虫分别置于内衬玻璃的微型孔或闪烁瓶中接触毒素。该方法可避免幼虫间因捕食导致的死亡。在世界卫生组织指南目前规定的每组25只幼虫的生物测定中，这种幼虫死亡情况很常见。我们的方法缩短了测试时间，提高了准确性，并改善了统计精度。
