Schnell D J, Pfannenstiel M A, Nickerson K W
Science. 1984 Mar 16;223(4641):1191-3. doi: 10.1126/science.6701520.
Solubilized crystals of Bacillus thuringiensis var. israelensis were 7000 times less toxic to Aedes aegypti larvae than intact crystals, presumably because mosquito larvae are filter feeders and selectively concentrate particles while excluding water and soluble molecules. A procedure is described whereby soluble toxins are adsorbed to 0.8-micrometer latex beads, with retention of toxicity. The latex bead assay should make it possible to analyze the structure and mode of action of the mosquito toxin.
苏云金芽孢杆菌以色列变种的溶解晶体对埃及伊蚊幼虫的毒性比完整晶体低7000倍，推测是因为蚊虫幼虫是滤食性动物，会选择性地浓缩颗粒，同时排除水和可溶性分子。本文描述了一种将可溶性毒素吸附到0.8微米乳胶珠上并保持毒性的方法。乳胶珠测定法应该能够分析蚊虫毒素的结构和作用方式。
