Richter Karin, Wolf Gerald, Engelmann Mario
Otto-von-Guericke-Universität, Institut für Medizinische Neurobiologie, D-39120 Magdeburg, Germany.
Learn Mem. 2005 Jul-Aug;12(4):407-13. doi: 10.1101/lm.97505.
Olfactory recognition memory was tested in adult male mice using a social discrimination task. The testing was conducted to begin to characterize the role of protein synthesis and the specific brain regions associated with activity in this task. Long-term olfactory recognition memory was blocked when the protein synthesis inhibitor anisomycin was injected 20 min before, immediately after, or 6 h after sampling. No effect was observed when anisomycin was administered 3 h or 18 h after sampling. Immunohistochemical analysis of Fos expression revealed that sampling-like exposure to a juvenile increased the activity of a subset of cells in the accessory olfactory bulb and the brain areas that are associated with it. Additionally, increased Fos expression was measured in the main olfactory bulb and the piriform cortex, whereas no signs of activation were seen in the cortical nucleus of the amygdala, all components of the main olfactory system. No increases in Fos immunoreactivity were observed after 4 h. Our data suggest that long-lasting olfactory recognition memory requires two stages of protein synthesis. The first stage takes place within 1-2 h and the second stage between 6-7 h after sampling. The first but not the second stage is paralleled by an increase in the number of Fos-immunoreactive cells in brain areas associated with both the main and accessory olfactory systems. It therefore appears that the role of the second stage of protein synthesis in recognition memory depends on the integrity of the first stage of protein synthesis.
使用社会辨别任务对成年雄性小鼠的嗅觉识别记忆进行了测试。进行该测试是为了开始表征蛋白质合成的作用以及与该任务中的活动相关的特定脑区。当在取样前20分钟、取样后立即或取样后6小时注射蛋白质合成抑制剂茴香霉素时，长期嗅觉识别记忆被阻断。在取样后3小时或l8小时给予茴香霉素时未观察到效果。对Fos表达的免疫组织化学分析显示，与幼年小鼠类似的取样暴露增加了副嗅球及其相关脑区中一部分细胞的活性。此外，在主嗅球和梨状皮层中检测到Fos表达增加，而在主嗅觉系统的所有组成部分杏仁核的皮质核中未观察到激活迹象。4小时后未观察到Fos免疫反应性增加。我们的数据表明，持久的嗅觉识别记忆需要两个蛋白质合成阶段。第一阶段在取样后1 - 2小时内发生，第二阶段在取样后6 - 7小时之间发生。第一阶段而非第二阶段与主嗅觉系统和副嗅觉系统相关脑区中Fos免疫反应性细胞数量的增加同时出现。因此，蛋白质合成第二阶段在识别记忆中的作用似乎取决于蛋白质合成第一阶段的完整性。
