Yang Wen-xiu, Li Gan-di, Zhou Qiao, Liu Wei-ping, Li Lei, Chen Ni
Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Jul;36(4):484-7.
To investigate the expression of API2-MALT1 fusion gene mRNA in Hashimoto's thyroiditis, thyroid MALT lymphoma and DLBCL, as well as the correlation between the expression and the pathogenesis and prognosis of two types of thyroid lymphoma.
Eight cases of Hashimoto's thyroiditis, 12 cases of MALT lymphoma and 3 cases of DLBCL in thyroid were selected and retrieved. API2-MALT1 transcripts were investigated in all cases by reverse transcript touchdown-PCR and nested PCR. The effect of API2-MALT1 fusion gene on the prognosis of thyroid lymphomas was assessed by analyzing the clinicopathological features and the follow-up findings.
The variant 1446/1123 transcript was detected in 2 of thyroid lymphomas (1 MALT lymphoma, 1 DLBCL) but not in 8 cases of Hashimoto's thyroiditis and 5 cases of chronic lymphadenitis. Both of API2-MALT1-positive cases were accompanied with Hashimoto's thyroiditis, showing stage I E and no involvement of lymph node.
The incidence of API2-MALT1 transcript in thyroid lymphomas was low; however, the correlation between API2-MALT1 fusion gene and the progression from Hashimoto's thyroiditis to lymphoma could not be excluded; the thyroid lymphoma with API2-MALT1 transcript seemed to develop on a more indolent clinical course.
探讨API2-MALT1融合基因mRNA在桥本甲状腺炎、甲状腺黏膜相关淋巴组织淋巴瘤（MALT淋巴瘤）及弥漫大B细胞淋巴瘤（DLBCL）中的表达情况，以及该表达与两种甲状腺淋巴瘤发病机制及预后的相关性。
选取8例桥本甲状腺炎、12例MALT淋巴瘤及3例甲状腺DLBCL病例并进行回顾性分析。采用逆转录降落式聚合酶链反应（RT-TD-PCR）和巢式聚合酶链反应（nested-PCR）检测所有病例中的API2-MALT1转录本。通过分析临床病理特征及随访结果评估API2-MALT1融合基因对甲状腺淋巴瘤预后的影响。
在2例甲状腺淋巴瘤（1例MALT淋巴瘤、1例DLBCL）中检测到变异体1446/1123转录本，而在8例桥本甲状腺炎及5例慢性淋巴结炎中未检测到。2例API2-MALT1阳性病例均伴有桥本甲状腺炎，表现为ⅠE期，无淋巴结受累。
API2-MALT1转录本在甲状腺淋巴瘤中的发生率较低；然而，不能排除API2-MALT1融合基因与桥本甲状腺炎向淋巴瘤进展之间的相关性；具有API2-MALT1转录本的甲状腺淋巴瘤似乎临床病程更为惰性。
