Liu Hui, Peng Zhi-lan, Wang He, Liu Shan-ling, Zhang Chong-shu, Tang Qian-ping, He Bin
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Jul;36(4):506-9.
To explore the relationship of loss of heterozygosity (LOH) and microsatellite instability (MI) of fragile histidine triad (FHIT) gene to the development of cervical carcinoma.
Two sites of microsatellite polymophism in FHIT gene were selected to detect LOH and MI in 60 cases of primary invasive cervical carcinoma and 35 cases of cervical intra-epithelial neoplasia (CIN).
At D3S1234 and D3S1300, the LOH rates of primary invasive cervical carcinomas were 45.0% (27/60) and 38.3% (23/60), the MI rates were 18.3% (11/60) and 11.7% (7/60), respectively. The LOH rates of CINs were 42.9% (15/35) and 37.1% (13/35), the MI rates were 11.4% (4/35), 8.6% (3/35), respectively. There were no significant differences between invasive cervical carcinomas and CINs in respect to their positive rates of LOH and MI at D3S1234 and D3S1300 (P>0.05). There were significant differences in LOH rates at D3S1234 and D3S1300 between the well/moderately differentiated cervical carcinomas and the poorly differentiated invasive cervical carcinomas (P<0.05). Significant differences were noted between the invasive cervical carcinomas with lymph node metastasis and those without lymph node metastasis in regard to their LOH and MI at the two sites (P < 0.05). The positive rates of LOH and MI for CIN III and noninvasive cervical carcinomas were higher than those for CIN I-II.
The FHIT gene change is a relatively late event in CINs. The detection of the LOH of FHIT gene might be helpful to the early diagnosis and the screening of cervical carcinoma. It might also be useful for predicting the prognosis of cervical carcinoma.
探讨脆性组氨酸三联体（FHIT）基因杂合性缺失（LOH）及微卫星不稳定性（MI）与宫颈癌发生发展的关系。
选取FHIT基因的两个微卫星多态性位点，检测60例原发性浸润性宫颈癌及35例宫颈上皮内瘤变（CIN）中的LOH及MI。
在D3S1234和D3S1300位点，原发性浸润性宫颈癌的LOH率分别为45.0%（27/60）和38.3%（23/60），MI率分别为18.3%（11/60）和11.7%（7/60）。CIN的LOH率分别为42.9%（15/35）和37.1%（13/35），MI率分别为11.4%（4/35）、8.6%（3/35）。原发性浸润性宫颈癌与CIN在D3S1234和D3S1300位点的LOH及MI阳性率差异无统计学意义（P>0.05）。高/中分化宫颈癌与低分化浸润性宫颈癌在D3S1234和D3S1300位点的LOH率差异有统计学意义（P<0.05）。有淋巴结转移与无淋巴结转移的浸润性宫颈癌在这两个位点的LOH及MI差异有统计学意义（P<0.05）。CINⅢ和非浸润性宫颈癌的LOH及MI阳性率高于CINⅠ-Ⅱ。
FHIT基因改变在CIN中是相对较晚发生的事件。检测FHIT基因的LOH可能有助于宫颈癌的早期诊断和筛查，也可能对预测宫颈癌预后有帮助。
