[经睾丸细针穿刺获取的冷冻复苏睾丸精子行卵胞浆内单精子注射]
[Intracytoplasmic sperm injection using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration].
作者信息
Teng Xiao-ming, Zhu Li-ping, Li Kun-ming, Wang Yu, Yao Hong-xia, Shao Jing-yu
机构信息
Assistant Reproductive Technology Center, Shanghai First Maternity and Infant Health Hospital, Shanghai 200040, China.
出版信息
Zhonghua Nan Ke Xue. 2005 Jul;11(7):517-9.
OBJECTIVE
To evaluate the effect of intracytoplasmic sperm injection (ICSI) using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration (TEFNA) in patients with non-obstructive azoospermia.
METHODS
Sixty-two patients underwent TEFNA. Mature testicular spermatozoa were found in 35 cases of patients and the testicular tissues were cryopreserved for later ICSI. Ovarian stimulation included a long protocol of GnRHa/FSH/hCG. Oocyte retrieval was performed under transvaginal ultrasound guidance, and ICSI conducted with cryopreserved-thawed testicular spermatozoa.
RESULTS
A total of 35 couples underwent 35 ICSI cycles using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration. The clinical pregnancy rate was 37.14% (13/35).
CONCLUSION
ICSI using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration is a main and effective method in the treatment of non-obstructive azoospermia, which can avoid further testicular fine needle aspiration.
目的
评估采用睾丸细针抽吸术（TEFNA）获取的冻融睾丸精子进行卵胞浆内单精子注射（ICSI）治疗非梗阻性无精子症患者的效果。
方法
62例患者接受了TEFNA。35例患者发现有成熟睾丸精子，将睾丸组织冷冻保存以备后续ICSI使用。卵巢刺激采用GnRHa/FSH/hCG长方案。在经阴道超声引导下取卵，并使用冻融睾丸精子进行ICSI。
结果
共有35对夫妇采用睾丸细针抽吸术获取的冻融睾丸精子进行了35个ICSI周期。临床妊娠率为37.14%（13/35）。
结论
采用睾丸细针抽吸术获取的冻融睾丸精子进行ICSI是治疗非梗阻性无精子症的一种主要且有效的方法，可避免进一步的睾丸细针抽吸术。
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